Praxilabs

OVERVIEW

- PCR is used to amplify a specific region of a DNA strand (DNA target). Most PCR methods typically amplify DNA fragments of up to ~10kb (our case).   A PCR setup requires several components and reagents. These components include:

  • Two primers (Forward and reverse).
  • Taq polymerase.
  • Deoxynucleoside triphosphates (dNTPs).
  • Buffer solution.
  • Distilled water.

-In the PCR experiment, the experiment begins with the preparation of the master mix of the above mentioned components. The second step in the experiment is adding a specific amount of the master mix to the DNA template that contains the DNA region (target) to be amplified that you previously prepared in the DNA extraction lab.

-After adding the master mix to the DNA template, the tube will be placed in the PCR device and the device will be set so that the DNA sample will pass by four stages inside the PCR device. These stages are:
  • Stage one: Initialization denaturation.
  • Stage two: Multiplication cycles.
  • Stage three: Final elongation.
  • Stage four: Final hold.

Aim

  • To amplify a specific region of a DNA strand (DNA target).

Techniques in Lab

  • Conventional Polymerase Chain Reaction

Real Screenshots of the Experiment

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Free access to sample experiments of Physics, Chemistry and Biology
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PraxiLabs is Designed to Fit Your Science Teaching Needs

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Tens of 3D simulations of science with you everywhere and all the time


You can easily access more than 130 of the 3D simulations of Physics, Chemistry and Biology anytime via your PC, laptop, tablet or even your smartphone, without any need to install an additional driver software.

Our virtual lab aims to facilitate the science teaching process for educators and also make it easy for students to absorb the theory and the application of all science experiments.

All of the 3D simulations are baked with a helping multimedia files to ensure that all learners can perform the science experiments without facing any difficulties.




Our virtual labs enable your institution to save most of physical labs budget


Save most of the money you always dedicate to spend on the materials and tools of the physical science labs. PraxiLabs will enable your students to repeat the steps of the experiments for unlimited times without any need to spend more money to get lab supplies.

Praxilabs will reduce the regular costs that allocated for the maintenance of the equipment of your physical labs as well as helps to increase its lifetime due to the lower frequency of usage.

You will also be able to teach your students how to perform the science experiments that requires a very expensive equipment without the need to purchase such equipment.

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Discover our Experiments

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DNA Extraction

- The isolation of the DNA involves breaking the cell and the nucleus to extract the DNA from within, then isolating it from the impurities, proteins,......

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Tests for Carboxylic Group

- Carboxylic acid contains the carboxyl functional group (-COOH). These acids turn a blue litmus paper to red. This group of compounds can be......

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Determination of Number of Particles of Water Crystallization in Borax

-Water of crystallization is the water forming part of the crystal structure of a certain substance, known as crystalline hydrates. Water of......

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