RNA Extraction Virtual Lab Simulation| PraxiLabs

RNA Extraction

Biology | Molecular Biology | Biochemistry | Genetics | Microbiology


As Featured In

What You Need to Know about RNA Extraction Virtual Lab

Importance of RNA Extraction

cellular RNA Extraction using Trizol reagent method.

RNA Extraction Experiment Method

Trizol reagent method.

RNA Extraction Virtual Lab - Learning Objectives for Students

  • <p>Demonstrate proficiency with the RNA extraction protocol. Identify the role of specific reagents and equipment in the RNA extraction virtual lab.

  • Practice basic laboratory techniques.

  • Conclude downstream applications of RNA extraction.</p>

RNA Extraction Virtual Lab & Theoretical Background to Know

The ‘central dogma’ of molecular biology illustrates the flow of genetic information from DNA, through RNAs, to proteins. There are several types of RNA in the cell. The messenger RNA (mRNA) is formed during the process of transcription from DNA, which basically means the production of a translatable copy of DNA, eventually leading to the synthesis of a specific protein. mRNA is then involved in the process of translation along with transfere RNA (tRNA) and ribosomal RNA (rRNA). tRNA is the amino acid carrier, it reads the code of the mRNA and adds the appropriate amino acid accordingly. 

rRNA is the factory where the process of translation occurs. Other RNAs include small and long non coding RNAs which are believed to have regulatory impact over gene expression. RNAs were also found to act as enzymes e.g. ribozymes. They sometimes carry the genetic material instead of DNA e.g. viral RNA. Studying RNA thus can be conducted for various reasons; for example, to study gene expression and its regulation. Detection of viral RNA can be used to diagnose infection.

What Is RNA Extraction Principle of Work?

In RNA extraction virtual lab, Total RNA is isolated and separated from DNA and protein after extraction with a solution called Trizol reagent. Trizol is an acidic solution containing guanidinium thiocyanate (GITC), phenol and chloroform. GITC irreversibly denatures the DNA which is a process in which proteins or nucleic acids lose their quaternary, tertiary and secondary structure, which are present in their native states. This is followed by centrifugation. Under acidic conditions, total RNA remains in the upper aqueous phase, while most of DNA and proteins remain either in the interphase or in the lower organic phase. Total RNA is then recovered by precipitation with isopropanol. Finally, RNA washing and purification is done using 70 % alcohol.


PraxiLabs is Recognized Worldwide

Customers Love PraxiLabs

“With the onset of the COVID-19 pandemic, we found ourselves in a situation that forced us to act quickly to find the best solution available to provide our students with a quality molecular genetics laboratory experience.”

Korri Thorlacius, B.Sc.
Biology Laboratory Instructor
Biology Department
Kwantlen Polytechnic University

'' Although there are now several vendors offering virtual reality software for physics labs, there is only one that offers a realistic, I feel like I’m in a real lab, solution: PraxiLabs.''

Dr.‌ ‌William‌ ‌H.‌ ‌Miner,‌ ‌Jr.‌ ‌
Professor‌ ‌of‌ ‌Physics‌ ‌
Palm‌ ‌Beach‌ ‌State‌ ‌College‌ ‌
Boca‌ ‌Raton,‌ ‌FL‌

" PraxiLabs offered my students a chance to actively engage with the material. Instead of watching videos on a topic, they could virtually complete labs and realize the practical applications of class topics. This is a quality alternative to in-person labs."

Crys Wright
Teaching Assistant
Texas A&M University, USA

"Great user experience and impressive interaction, I am very pleased to have tried the simulations and will continue to do so."

Dr. Khaled M Goher
Lecturer in Biomedical Engineering
Aston University, UK

The #1 Science Virtual Labs used by Educational Institutions

Explore More Interactive 3D Virtual Simulations

Designed for Safety and Engagement

Find out how PraxiLabs keeps students engaged and improves learning outcomes