{"id":1947,"date":"2022-08-24T17:16:55","date_gmt":"2022-08-24T17:16:55","guid":{"rendered":"https:\/\/blog.praxilabs.com\/?p=1947"},"modified":"2025-10-19T18:16:20","modified_gmt":"2025-10-19T18:16:20","slug":"7-different-dna-extraction-methods","status":"publish","type":"post","link":"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/","title":{"rendered":"7 Different DNA Extraction Methods"},"content":{"rendered":"<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">DNA extraction is one of the basic techniques employed in the molecular laboratory, done for the first time by Friedrich Miescher in 1869.<\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">In this article, we will focus on DNA extraction methods, comparing methods for more understanding. Along with this, we will explain DNA extraction protocol,<\/span> <span style=\"font-weight: 400;\">DNA extraction experiment, and advantages DNA extraction methods.<\/span><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0Although Friedrich Miescher isolated the cell material \u201cnuclei\u201d or \u201cnucleic acid\u201d and developed a method for nucleic acid isolation, he was not sure whether what he isolated was DNA or not.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">In 1958 Stahl and Meselson developed a full-function protocol for DNA extraction (The density gradient centrifugation protocol) that was the first protocol described for isolating DNA from E.coli bacteria.<\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\"><strong><a class=\"maxbutton-3 maxbutton\" href=\"https:\/\/praxilabs.com\/en\/3d-simulations\/dna-extraction-virtual-lab-biology-simulation\"><span class='mb-text'>Try DNA Extraction Now For FREE<\/span><\/a><\/strong><\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-1948 size-full aligncenter\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2022\/08\/dna_precipitate_large20161208-1536-19xsgs6.jpg\" alt=\"DNA Extraction\" width=\"500\" height=\"375\" \/><\/span><\/p>\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_82_2 counter-hierarchy ez-toc-counter ez-toc-light-blue ez-toc-container-direction\">\r\n<div class=\"ez-toc-title-container\">\r\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\r\n<span class=\"ez-toc-title-toggle\"><\/span><\/div>\r\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#What_is_DNA_Extraction\" >What is DNA Extraction ?<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#What_is_DNA_Extraction_Principle\" >What is DNA Extraction Principle?<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#Different_Types_of_DNA_Extraction_Methods\" >Different Types of DNA Extraction Methods<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#Create_FREE_Account_To_Try_The_Experiment_Now\" >Create FREE Account To Try The Experiment Now<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#Chemical-based_DNA_extraction_method\" >Chemical-based DNA extraction method<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#Physical_or_Solid-Based_DNA_Extraction_Methods\" >Physical or Solid-Based DNA Extraction Methods\u00a0<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#Liquid-Liquid_DNA_Extraction\" >Liquid-Liquid DNA Extraction\u00a0<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#Comparison_of_DNA_Extraction_Methods\" >Comparison of DNA Extraction Methods<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#Proteinase_K_DNA_Extraction\" >Proteinase K DNA Extraction<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#The_Salting-out_DNA_Extraction\" >The Salting-out DNA Extraction<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-11\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#SDS_DNA_Extraction\" >SDS DNA Extraction<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-12\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#CTAB_DNA_Extraction\" >CTAB DNA Extraction<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-13\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#Silica_Gel_Based_Techniques\" >Silica Gel Based Techniques<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-14\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#Home_DNA_extraction_How_to_Extract_Your_Own_DNA_at_Home\" >Home DNA extraction | How to Extract Your Own DNA at Home<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-15\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#Advantages_of_DNA_Extraction_Methods\" >Advantages of DNA Extraction Methods<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-16\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#DNA_Extraction_Experiment_from_PraxiLabs\" >DNA Extraction Experiment from PraxiLabs<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-17\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#The_Procedures_Of_DNA_extracted_virtual_labs\" >The Procedures Of DNA extracted virtual labs<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-18\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#DNA_Isolation\" >DNA Isolation<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-19\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#DNA_Extraction_Done_Right_A_Guide_to_Safe_and_Responsible_DNA_Extraction_Practices\" >DNA Extraction Done Right: A Guide to Safe and Responsible DNA Extraction Practices<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-20\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#Factors_to_consider_while_choosing_the_suitable_method_for_your_DNA_extraction\" >Factors to consider while choosing the suitable method for your DNA extraction:<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-21\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#DIY_DNA_extraction_Home_DNA_extraction\" >DIY DNA extraction | Home DNA extraction<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-22\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#What_is_the_DNA_extraction_process\" >What is the DNA extraction process?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-23\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#What_are_the_two_main_types_of_DNA_extraction\" >What are the two main types of DNA extraction?<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-24\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#What_are_the_three_basic_steps_for_DNA_extraction\" >What are the three basic steps for DNA extraction?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-25\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#What_are_five_facts_about_DNA_extraction\" >What are five\u00a0 facts about DNA extraction?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-26\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#What_are_some_challenges_in_extracting_DNA\" >What are some challenges in extracting DNA?<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-27\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/08\/24\/7-different-dna-extraction-methods\/#PraxiLabs_Molecular_biology_simulations\" >PraxiLabs Molecular biology simulations<\/a><\/li><\/ul><\/nav><\/div>\r\n<h2><span class=\"ez-toc-section\" id=\"What_is_DNA_Extraction\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>What is DNA Extraction ?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">DNA extraction is a process that used to purify DNA by using chemical or physical methods from a biological sample separating DNA from protein, cell membranes, and other cellular components.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">DNA extraction protocol involves lysis of membranes for DNA extraction, then separating DNA from impurities like proteins, and other substances.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"What_is_DNA_Extraction_Principle\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>What is DNA Extraction Principle?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">DNA extraction involves lysis of cellular and nuclear membranes in order to extract DNA from within. This is followed by DNA separation from impurities, proteins, and other substances.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0General Stages of DNA Extraction are:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Cell dissolution: lysis of cell and the nucleus to extract DNA into the buffer.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Precipitation: removing the impurities and proteins from the sample.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Purification: This final stage is done to get a completely pure DNA sample ready to be used.<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Note: the main steps remain similar among all DNA extraction methods.<\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">For more information about DNA extraction, visit our article &#8220;<a href=\"https:\/\/praxilabs.com\/en\/blog\/2020\/06\/30\/dna-extraction-virtual-lab\/\">DNA Extraction<\/a> Virtual Lab Experiment from PraxiLabs&#8221;<\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4384 size-full\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/06\/7-Different-DNA-Extraction-Methods-3.webp\" alt=\"Different Types of DNA Extraction Methods\" width=\"653\" height=\"355\" srcset=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/06\/7-Different-DNA-Extraction-Methods-3.webp 653w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/06\/7-Different-DNA-Extraction-Methods-3-300x163.webp 300w\" sizes=\"auto, (max-width: 653px) 100vw, 653px\" \/><\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Different_Types_of_DNA_Extraction_Methods\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Different Types of DNA Extraction Methods<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Depending upon the sample type, every DNA extraction method varies (there are d<\/span>ifferent ways to extract DNA<span style=\"font-weight: 400;\">), for example, the DNA extraction method for plant DNA is different from <\/span>DNA extracted from blood\u00a0<span style=\"font-weight: 400;\">. Likewise, the bacterial DNA isolation method is different from other types. So, varieties of DNA extraction methods are needed for different samples. Let&#8217;s c<\/span>ompare and contrast different DNA extraction methods and then you can choose the best DNA extraction method for your project!<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">DNA extraction methods are broadly categorized into:<\/span><\/p>\n<ol>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\"> \u00a0 <\/span> <span style=\"font-weight: 400;\">Chemical-based (or solution- based) DNA extraction methods.<\/span><\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\"> \u00a0 <\/span> <span style=\"font-weight: 400;\">Solid-phase DNA extraction methods (Physical method).<\/span><\/span><\/li>\n<\/ol>\n<h3 style=\"text-align: center;\"><span class=\"ez-toc-section\" id=\"Create_FREE_Account_To_Try_The_Experiment_Now\"><\/span><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b><a class=\"maxbutton-3 maxbutton\" href=\"https:\/\/praxilabs.com\/en\/sign-up\"><span class='mb-text'>Create FREE Account To Try The Experiment Now<\/span><\/a><\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<h2><span class=\"ez-toc-section\" id=\"Chemical-based_DNA_extraction_method\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Chemical-based DNA extraction method<\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The Chemical or solution-based method uses many organic and inorganic solutions. Chemicals like phenol, chloroform, CTAB Triton X100, SDS,\u00a0 isoamyl alcohol, Tris and EDTA are used in the chemical -based DNA extraction method.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The solution-based or chemical-based DNA extraction method is subdivided into:<\/span><\/p>\n<ul>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Organic solvent-based DNA extraction<\/span><\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">This method depends on the use of organic substances such as phenol and chloroform.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Example: Phenol-chloroform and isoamyl alcohol<\/span><\/p>\n<ul>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">\u00a0<\/span><span style=\"font-weight: 400;\">Inorganic solvent-based DNA extraction<\/span><\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">It depends on the inorganic solvents.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Examples: \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 \u00a0 <\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Proteinase K DNA extraction.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Salting out method.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">SDS DNA extraction.<\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">CTAB DNA extraction.<\/span><\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Silica-gel-based techniques.<\/span><\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"Physical_or_Solid-Based_DNA_Extraction_Methods\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Physical or Solid-Based DNA Extraction Methods\u00a0<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Paper DNA extraction.\u00a0<\/span><\/li>\n<li aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Magnetic bead DNA extraction.<\/span><\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"Liquid-Liquid_DNA_Extraction\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Liquid-Liquid DNA Extraction\u00a0<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Liquid-liquid extraction is one of the commonest methods for nucleic acid extraction. In this method the solutions prepared by various chemical compositions are used for extraction and it mainly relies on lysis buffer preparation.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Lysis buffer is prepared in one or two solutions as it uses many chemicals. Common chemicals that used for liquid-liquid DNA extraction are Phenol, chloroform, isoamyl alcohol, CTAB, SDS, Tris, EDTA, MgCl2, and other detergents.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">These methods commonly require centrifugation for separation. Examples of liquid-liquid DNA extraction methods: Phenol, chloroform and isoamyl alcohol DNA extraction, SDS DNA extraction and CTAB DNA extraction.\u00a0\u00a0<\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4385 size-full\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/06\/7-Different-DNA-Extraction-Methods-4.webp\" alt=\"organic phenol chloroform\" width=\"656\" height=\"275\" srcset=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/06\/7-Different-DNA-Extraction-Methods-4.webp 656w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/06\/7-Different-DNA-Extraction-Methods-4-300x126.webp 300w\" sizes=\"auto, (max-width: 656px) 100vw, 656px\" \/><\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Comparison_of_DNA_Extraction_Methods\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Comparison of DNA Extraction Methods<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Phenol-Chloroform Method<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">In this method we can separate the DNA based on the solubility of DNA molecules in immiscible solutions, and that is called the liquid-liquid DNA extraction methods (the phenol chloroform DNA extraction method is one of these methods).<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Although this method is considered one of the best methods, it is less recommended because of phenol and chloroform harmful. The quantity and quality of DNA obtained by method are very good.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">This method is known as a phenol-chloroform and isoamyl alcohol or PCI method of DNA extraction.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The major chemicals of PCI DNA extraction methods are Phenol, chloroform and lysis buffer (contains EDTA, Tris, NaCl, MgCl2, SDS, and other salts).<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The organic component of the technique- phenol and chloroform denature the protein portion of cells and lysis buffer components help in the cell membrane and nuclear lysis.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The lysis buffer contains salts like\u00a0 EDTA, NaCl, Tris and SDS.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">In principle, the phenol digests proteins, isoamyl alcohol separates nucleic acid and chloroform reduces the foaming between interphase.\u00a0\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The main advantage of the PCI is that it can isolate DNA from nearly all tissue types (It works for animal, plant and bacterial cells) and it can isolate RNA when used with guanidine thiocyanate.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Unfortionatly, this method is it is unsafe because Phenol is volatile and can cause burns. the chloroform can faint us. So, it requires training and to prepare and handle chemicals.\u00a0<\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>PraxiLabs provides the DNA extraction experiment at our <a href=\"https:\/\/praxilabs.com\/en\/virtual-biology-lab\">biology virtual lab<\/a> that based on the phenol chloroform extraction method &#8230; <\/b><a href=\"https:\/\/praxilabs.com\/en\/pricing\"><b>Subscribe and try it now<\/b><\/a><\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Proteinase_K_DNA_Extraction\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Proteinase K DNA Extraction<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The proteinase K-based method is one of inorganic solution-based technique for DNA extraction.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The proteinase K DNA extraction method provides high quantity of DNA but thee disadvantage of this method is that takes long time. Also, in case of not maintained well in a cold chain, the enzyme proteinase K cannot be utilized for a longer period of time. Another major problem is that The stability of the enzyme is low.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4386 size-full\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/06\/7-Different-DNA-Extraction-Methods-5.webp\" alt=\"The Salting-out DNA Extraction\" width=\"1024\" height=\"576\" srcset=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/06\/7-Different-DNA-Extraction-Methods-5.webp 1024w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/06\/7-Different-DNA-Extraction-Methods-5-300x169.webp 300w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/06\/7-Different-DNA-Extraction-Methods-5-768x432.webp 768w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"The_Salting-out_DNA_Extraction\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>The Salting-out DNA Extraction<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Extraction method is a simple and non-toxic technique that isolates a high-quality DNA from the whole blood. In the standard salting-out method, proteins K and RNase are added to them after the lysis of cells. Saturated NaCl was needed for the proteins to precipitate out of the solution. The cell samples are centrifuged and then,\u00a0 DNA is separated by washing it with detergent like ethanol.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The salting-out DNA extraction method is safer than the phenol-chloroform method. It depends on the usage of salts such as potassium acetate, sodium chloride, and ammonium acetate that help in DNA extraction.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">This method gives excellent results in combination with proteinase K and enough yield can be obtained but the quality (purity) obtained might not be good.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"SDS_DNA_Extraction\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>SDS DNA Extraction<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">SDS or sodium dodecyl sulfate is an anionic detergent that digests nuclear and cell membrane proteins.\u00a0 \u00a0 <\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">SDS gives a negative charge to each protein as a function of their size. Because all of proteins have the same shape in the gel separation they are separated only for their size. So, SDS can be used to aid in lysing cell during DNA extraction.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"CTAB_DNA_Extraction\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>CTAB DNA Extraction<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">CTAB or Cetyl Trimethyl Ammonium Bromide is a chemical often used in DNA extraction. This method is a specially prepared liquid-liquid and solution-based extraction method for plant DNA extraction.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The CTAB buffer removes polysaccharides and polyphenols effectively and gives excellent yield for plant DNA.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">This method required extensive chemical preparation and additional techniques like tissue homogenization and the use of liquid nitrogen. It also takes a long time.<\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Silica_Gel_Based_Techniques\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Silica Gel Based Techniques<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">In the spin-column DNA extraction technique, a silica gel as a solid phase is immobilized in a tube. A specially prepared lysis buffer is used to digest the sample and allows it to separate on a solid phase. the sample is Centrifuged first to remove all the debris by protecting the DNA and changing the pH of the solution(to elutes DNA in the last step). these techniques are now commercially available.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Spin column is the most advanced, effective, rapid and accurate separation technique. It is, however, a chemical method of DNA extraction but works on the principle of solid-phase separation.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The main advantage of this technique is that can isolate DNA from any biological sample and tissue. However, it is difficult to isolate DNA from plants even using the spin-column technique, Different columns are now available depending upon the sample type.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">But this technique is Cost, lacks optimizations and the yield remains comparatively low but good.\u00a0<\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Home_DNA_extraction_How_to_Extract_Your_Own_DNA_at_Home\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Home DNA extraction | How to Extract Your Own DNA at Home<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Let&#8217;s try one of simple DNA extraction methods (DIY DNA extraction)!<\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>To do DNA Extraction experiment at home you will need:<\/b><\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Zip lock bag (heavy duty).<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">3 fresh strawberries.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">1 pinch of\u00a0 table salt (NaCl).<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">8 drops of\u00a0 shampoo (without conditioner).<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Strainer.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Ice- cold isopropyl rubbing alcohol.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Small clear glass or test tube.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Large paper clip, straightened out.<\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Steps:<\/b><\/span><\/p>\n<ol>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Wash the strawberries and remove the sepals (the green leaves)<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Place the strawberries in a zip lock plastic bag and crush it with your fist<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Add the salt and shampoo to the mashed strawberry mixture in the bag, zip it up and squeeze it in your hands for 1 minute.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Place the strainer over the small glass.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Pour the strawberry shampoo mixture into the strainer. Filter the mixture into the glass.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Slowly pour ice cold rubbing alcohol into the glass, until it is about half full.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The alcohol will form a separate layer on top of the strawberry filtrate.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Keep the glass still at eye level; do not shake it. Watch what happens. DNA will begin to appear where the alcohol and filtrate layer meet.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Carefully scoop out the DNA with the straightened end of the paper clip.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Now you can observe what the DNA looks like and what\u00a0 it feels like<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Discard the mashed strawberries and solution. Wash your equipment and clean your experiment area, and wash your hands!<\/span><\/li>\n<\/ol>\n<h2><span class=\"ez-toc-section\" id=\"Advantages_of_DNA_Extraction_Methods\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Advantages <\/b><b>of <\/b><b>DNA Extraction Methods<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4387 size-full aligncenter\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/06\/7-Different-DNA-Extraction-Methods-6.webp\" alt=\"Advantages of DNA Extraction Methods\" width=\"276\" height=\"183\" \/><\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">DNA extraction is vital to biology, especially biotechnology. It is the first step of different applications like fundamental research, disease diagnosis and therapeutic decision.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">One of the main advantages of DNA extraction methods is that they are very importance to define the unique characteristics of DNA like the shape, the size and function.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0DNA extraction is used in medical conditions investigation such as Down syndrome and cystic fibrosis. It is also helpful in case of identification if a person is a carrier of the disease or not.\u00a0 So, by DNA sequence in relation to diseases, It helped in finding out the molecular basis and cure for various diseases.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0In criminal investigations, DNA extraction from samples (e.g., hair &#8211; skin &#8211; blood) is used to determine if a person is a suspect or not and also it can prove whether a person was in the vicinity of the crime scene.<\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">It also used in<\/span> <span style=\"font-weight: 400;\">Paternity Tests.<\/span><\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0It can be useful in <a href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/06\/29\/genetic-engineering-in-humans-2\/\">genetic engineering<\/a>. For animals, DNA extraction is helpful for transforming and cloning animal\u2019s DNA. For plants, DNA can be useful in identifying and extracting a specific gene in order to replicate in\u00a0 generations of plants.<\/span><\/li>\n<\/ul>\n<ul>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">DNA study also helped in creating many vaccines (e.g.,<\/span> <span style=\"font-weight: 400;\">Hepatitis B vaccine), hormones (e.g., growth hormones and insulin), and enzymes.<\/span><\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">We can use extracted DNA for PCR experiments, DNA can be amplified with the help of PCR.<\/span><\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"DNA_Extraction_Experiment_from_PraxiLabs\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>DNA Extraction Experiment from PraxiLabs<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><a href=\"https:\/\/praxilabs.com\/\">PraxiLabs<\/a> provides DNA extraction lab that used to extract cellular DNA using phenol\/chloroform method.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The following video shows the steps of DNA extraction in the virtual lab:<\/span><\/p>\n<p style=\"text-align: center;\"><iframe loading=\"lazy\" src=\"\/\/www.youtube.com\/embed\/lfpYBM25Qsc?feature=emb_imp_woyt\" width=\"560\" height=\"314\" allowfullscreen=\"allowfullscreen\"><\/iframe><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">By the end of the experiment, students are able to understand one of the DNA extraction methods as well as efficiently deal with the protocol involved in DNA extraction in a safe environment with the ability to repeat the experiment an unlimited number of times at any time and from anywhere.<\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"The_Procedures_Of_DNA_extracted_virtual_labs\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400; font-size: 14pt;\"><b>The Procedures Of <\/b><\/span><span style=\"font-weight: 400; font-size: 14pt;\"><b>DNA extracted virtual labs<\/b><\/span><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">DNA extraction lab from PraxiLabs depends on one of the most popular <\/span>DNA isolation techniques<span style=\"font-weight: 400;\"> that includes the following steps:<\/span><\/span><\/p>\n<ol>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Prepare 10 microliters of RNAse enzyme and add it to the sample .<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Add 700 microliters of cell lysis buffer to the sample.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Place the sample in the water bath at 37 degrees Celsius for an hour.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Add 10 microliters of the Proteinase K enzyme and mix it with the sample several times with the pipette.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Mix the sample well with the vortexer for 15 seconds.\u00a0 (Note: Avoid creating bubbles in the sample.)<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Place the sample in the water bath at a temperature of 56 degrees Celsius for two hours.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Add 700 microliters of Phenol, Chloroform,and isoamyl alcohol in the ratios of 25:24:1, and pipet up and down several times to mix the solution with the sample using a pipette.\u00a0<\/span><\/li>\n<\/ol>\n<h3><span class=\"ez-toc-section\" id=\"DNA_Isolation\"><\/span><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">DNA Isolation<\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ol start=\"8\">\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Place the sample in the centrifuge and set it to 13,000 rpm for 10 minutes at a temperature of 4 degrees Celsius to isolate the contents of the tube to two layers. (Note: To balance the centrifuge a tube of the same size filled with water must be added to the opposite side of the sample tube.)<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Remove the top layer using the pipette, then place it in a clean tube.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Repeat steps 7, 8, and 9 twice.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Add 700 microliters of Chloroform to the sample you have removed.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Place the sample in the centrifuge and set it to 13,000 rpm for 10 minutes at a temperature of 4 degrees celsius to isolate the contents of the tube to two layers. (Note: To balance the centrifuge a tube of the same size filled with water must be added to the opposite side of the sample tube.)<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Remove the top layer using the pipette, then place it in the clean tube.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Repeat steps 12 and 13 once more .<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Add a solution of sodium acetate with a concentration of 3 Molar, a tenth of the volume of the sample, to the sample.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Add an ethanol solution with a concentration of 95%, twice the volume of the sample, to the sample.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Mix the contents of the tube by inverting it. Then leave it to precipitate at a temperature of &#8211; 70 degrees Celsius for a whole day.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Place the sample in the centrifuge and set it to 13,000 rpm for 30 minutes at a temperature of 4 degrees Celsius. (Note: To balance the centrifuge a tube of the same size filled with water must be added to the opposite side of the sample tube.)<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Pour the supernatant into a wastebasket. Retrieve the remaining material using a pipette gently so as\u00a0 not disturb the pellet.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Add 700 microliters of 70% ethanol. Then mix it by flicking it gently to prevent damage.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Place the sample in the centrifuge and set it to 13,000 rpm for 10 minutes at a temperature of 4 degrees Celsius. (Note: To balance the centrifuge a tube of the same size filled with water must be added to the opposite side of the sample tube.)<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Pour the liquid into the wastebasket, and the remaining is removed using the pipette gently to not disturb the pellet.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Dry the sample in the air.<\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\"> Dissolve the DNA sample in <\/span><b>TE buffer<\/b><span style=\"font-weight: 400;\"> and mix it with the sample several times using the pipette.Note: Now you have a DNA sample and can use it in other operations<\/span><\/span><\/li>\n<\/ol>\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><strong>Try the virtual lab of educational <a href=\"https:\/\/praxilabs.com\/en\/3d-simulations\/dna-extraction-virtual-lab-biology-simulation\" target=\"_blank\" rel=\"noopener\">DNA extraction simulation<\/a> now<\/strong><\/span><\/p>\n<h2 style=\"text-align: left;\"><span class=\"ez-toc-section\" id=\"DNA_Extraction_Done_Right_A_Guide_to_Safe_and_Responsible_DNA_Extraction_Practices\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">DNA Extraction Done Right: A Guide to Safe and Responsible DNA Extraction Practices<\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Wear appropriate protective clothing.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Keep biological agents in the correct areas to minimize exposure to risk.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0Disinfect work surfaces and properly dispose of waste.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Prevent the spread of contamination or spills.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Make sure that you know the importance of sanitizing your desk space as it is an effective way to ensure a clean and safe work environment.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Wear the laboratory coat while working in the lab.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0Avoid consuming or storing food and beverages in the laboratory.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0Make sure that you know the location of and how to use emergency equipment, including safety showers, eyewash stations, and medical cabinet.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0Ensure that your work area has proper ventilation to prevent the build-up of unwanted or harmful substances.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Cleaning the work area with medical-grade sanitizing products is important.<\/span><\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"Factors_to_consider_while_choosing_the_suitable_method_for_your_DNA_extraction\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Factors to consider while choosing the suitable method for your DNA extraction:<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>You can choose the best DNA extraction method for your project by checking\u00a0 the following factors:<\/b><span style=\"font-weight: 400;\">\u00a0<\/span><\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>The type of Sample: <\/b><span style=\"font-weight: 400;\">each type of sample (Bacteria, animal tissue , plants , water, blood , soil, saliva) requires a specific method to extract the DNA. Your type of sample is in fact the most crucial factor to consider when selecting a DNA extraction method.<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Plasmid DNA vs Genomic DNA: <\/b><span style=\"font-weight: 400;\">The <\/span><b>protocol <\/b><span style=\"font-weight: 400;\">for <\/span><b>plasmid DNA isolation<\/b><span style=\"font-weight: 400;\"> is different from the one used to isolate the genomic DNA. The difference is mainly in the lysis process, and some buffer formulations.<\/span><\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Downstream applications: <\/b><span style=\"font-weight: 400;\">The quality and purity of extracted DNA\u00a0 must fulfill the requirements of the downstream applications.<\/span><\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Sample quantity: <\/b><span style=\"font-weight: 400;\">Some methods are designed specifically for a certain amount of sample.<\/span><\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Yield: <\/b><span style=\"font-weight: 400;\">The DNA extraction method you choose should meet the yield expectations for your purpose.\u00a0<\/span><\/span><\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"DIY_DNA_extraction_Home_DNA_extraction\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>DIY DNA extraction | Home DNA extraction<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The following method is one of the easy DNA extraction methods for beginners, you will need DNA sources such as Spinach, Chicken liver, Strawberries, or Broccoli.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">In this experiment we will use green split peas.<\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Step 1: Blender Insanity!<\/b><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Put in a blender:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">1\/2 cup of split peas (100ml).<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">1\/8 teaspoon of table salt (less than 1ml).<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">1 cup cold water (200ml).<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Blend on high for 15 seconds. The blender separates the pea cells from each other, so you now have a very thin pea-cell soup.<\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Step 2: Soapy Peas<\/b><\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Pour your thin pea-cell soup through a strainer into another container (like a measuring cup).<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Add 2 tablespoons of liquid detergent (about 30ml) and swirl to mix.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Let the mixture sit for 5-10 minutes.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Pour the mixture into test tubes or other small glass containers, each about 1\/3 full.<\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Step 3: Enzyme Power<\/b><\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Add a pinch of enzymes to each test tube and stir gently. Be careful! If you stir too hard, you&#8217;ll break up the DNA, making it harder to see.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Use meat tenderizer for enzymes. If you can&#8217;t find tenderizer, try using pineapple juice or contact lens cleaning solution.<\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Step 4: Alcohol Separation<\/b><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Tilt your test tube and slowly pour rubbing alcohol (70-95% isopropyl or ethyl alcohol) into the tube down the side so that it forms a layer on top of the pea mixture. Pour until you have about the same amount of alcohol in the tube as pea mixture.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Alcohol is less dense than water, so it floats on top. Look for clumps of white stringy stuff where the water and alcohol layers meet.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The salt that you added in step one helps it stick together. So what you see are clumps of tangled DNA molecules!<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">DNA normally stays dissolved in water, but when salty DNA comes in contact with alcohol, it becomes undissolved. This is called precipitation. The physical force of the DNA clumping together as it precipitates pulls more strands along with it as it rises into the alcohol.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">You can use a wooden stick or a straw to collect the DNA. If you want to save your DNA, you can transfer it to a small container filled with alcohol.<\/span><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">DNA Extraction Demystified: Common Questions Answered!<\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"What_is_the_DNA_extraction_process\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>What is the DNA extraction process?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The extraction or isolation of DNA is the process of breaking open the cell and the nucleus to extract the DNA from within, then isolating it from\u00a0 impurities, proteins, and other substances.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The main stages of DNA Extraction involve:<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Cell lysis: In this stage the cell and the nucleus are broken open to extract a DNA sample.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Precipitation: In this stage, proteins and impurities are removed from the sample.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0Purification: This stage involves isolating the DNA completely from the other substances, for a pure DNA sample.<\/span><\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"What_are_the_two_main_types_of_DNA_extraction\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>What are the two main types of DNA extraction?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The two main types of DNA extraction are:<\/span><\/p>\n<ol>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0\u00a0Chemical-based (or solution- based) DNA extraction methods.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">\u00a0\u00a0Physical DNA extraction methods (solid-phase DNA extraction methods).<\/span><b>\u00a0<\/b><\/span><\/li>\n<\/ol>\n<h3><span class=\"ez-toc-section\" id=\"What_are_the_three_basic_steps_for_DNA_extraction\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>What are the three basic steps for DNA extraction?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Cell lysis: In this stage, the cell and the nucleus are broken open\u00a0 to extract a DNA sample.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Precipitation: In this stage, proteins and impurities are removed from the sample.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0Purification: This stage involves isolating the DNA completely from the other substances, resulting in a pure DNA sample.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"What_are_five_facts_about_DNA_extraction\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>What are five\u00a0 facts about DNA extraction?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ul>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">If all the DNA stored within your body was placed side by side in a chain, it would be 600 times longer than the distance from the Earth to the Sun and back.<\/span><\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Scientists did not know DNA contained genes until 1943. Prior to that ,they believed that genes were stored in proteins.<\/span><\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Human DNA is 98% identical to that of a chimpanzee while it is 40-50% identical to that of a cabbage.<\/span><\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Humans share 99.9% of their DNA code. The remaining 0.1% is responsible for the differences between us.<\/span><\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">If you could write 60 words per minute, and you continued to work 8 hours a day, it would take 50 years to write the human genome.<\/span><\/span><\/li>\n<\/ul>\n<h3><span class=\"ez-toc-section\" id=\"What_are_some_challenges_in_extracting_DNA\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>What are some challenges in extracting DNA?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<ul>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Not enough DNA quantity.<\/span><\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Samples not mixed well enough during extraction.<\/span><\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Proteinase K enzyme is inactive because it was prepared too far in advance.<\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Water baths are not at the proper temperature.<\/span><\/span><\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"PraxiLabs_Molecular_biology_simulations\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>PraxiLabs Molecular biology simulations<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">PraxiLabs simulations provide several features for more personalized learning such as:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>\u00a0The progress report<\/b><span style=\"font-weight: 400;\">: allows teachers to see how well their students are doing, track their progress in each experiment, and gain insights into their performance. It also shows any stages that have been skipped during the experiment.<\/span><\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">\u00a0For example: <\/span><a href=\"https:\/\/praxilabs.com\/en\/3d-simulations\/dna-extraction-virtual-lab-biology-simulation\"><span style=\"font-weight: 400;\">DNA extraction<\/span><\/a><span style=\"font-weight: 400;\"> has 3 stages. The feature captures any stages that have been skipped by the student in each attempt. It helps to know the number of times the student has asked our lab partner \u201cOxi\u201d to know the next step and the last step that the student stopped at, helping to identify students who may be struggling with certain steps or who might need more help in order to complete the experiment.<\/span><\/span><\/p>\n<ul>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Input for Pipette &amp; Observation Table:\u00a0 <\/b><span style=\"font-weight: 400;\">You can get the support you need instantly, if you forgot the right amount you need to enter or if data is entered incorrectly ( e.g., for the pipette or the observation table, at any point. The virtual assistant Oxi will alert you to any mistakes.<\/span><\/span><\/li>\n<\/ul>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Skipping ahead: <\/b><span style=\"font-weight: 400;\">Brings students a more user-centered, focused, and personalized learning experience. Students can skip certain experiment steps if they are already familiar with them. A list of the experiment milestones will allow them to select and focus on the stages they want to understand better.\u00a0<\/span><\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">We provide a diverse catalog of virtual biology practical simulations, allowing students to practice the same virtual biology practical simulation\u00a0 an unlimited number of times, with 0% risk and 100% supervision, <\/span><b>across various sub-disciplines of biology<\/b><span style=\"font-weight: 400;\">:<\/span><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Here are some examples of molecular biology virtual lab experiments available:<\/span><\/p>\n<ul>\n<li><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\"><a href=\"https:\/\/praxilabs.com\/en\/3d-simulations\/dna-extraction-virtual-lab-biology-simulation\">DNA Extraction<\/a><\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\">RNA Extraction<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\"><a href=\"https:\/\/praxilabs.com\/en\/3d-simulations\/cdna-synthesis-virtual-lab-biology-simulation\">cDNA Synthesis<\/a><\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\">Conventional PCR<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\"><a href=\"https:\/\/praxilabs.com\/en\/3d-simulations\/real-time-pcr-virtual-lab-simulation\">Real Time PCR<\/a><\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\">Agarose Gel Electrophoresis<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\">DNA fingerprinting using <a href=\"https:\/\/praxilabs.com\/en\/3d-simulations\/dna-fingerprinting-using-gel-electrophoresis-simulation\">Gel Electrophoresis<\/a><\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\">Bacterial Plating Out Technique Experiment <a href=\"https:\/\/praxilabs.com\/en\/3d-simulations\/streak-plate-method-virtual-lab-simulation\">(streak plate method)<\/a><\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\">Antibiotic Sensitivity Test Disc Diffusion Method<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\">Coagulase Test<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\"><a href=\"https:\/\/praxilabs.com\/en\/3d-simulations\/catalase-test-virtual-lab-simulation\">Catalase Test<\/a><\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\">Oxidase Test<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\">Indole Test<\/span><\/li>\n<li><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\">Spread Plate Technique<\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">\u00a0<\/span><b>2D Protein Electrophoresis(Isoelectric Point Focusing,PAGE)\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0<\/b><\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Protein Electrophoresis (Polyacrylamide Gel Electrophoresis-PAGE)<\/b><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0<b>On PraxiLabs, you can find different virtual biology simulations accessible anytime and anywhere.\u00a0<\/b><a href=\"https:\/\/praxilabs.com\/en\/sign-up\"><b>Subscribe and Get Started Now!<\/b><\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>DNA extraction is one of the basic techniques employed in the molecular laboratory, done for the first time by Friedrich Miescher in 1869. In this article, we will focus on DNA extraction methods, comparing methods for more understanding. Along with this, we will explain DNA extraction protocol, DNA extraction experiment, and advantages DNA extraction methods. &hellip;<\/p>\n","protected":false},"author":8,"featured_media":4382,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_lmt_disableupdate":"no","_lmt_disable":"no","footnotes":""},"categories":[6,9],"tags":[],"class_list":["post-1947","post","type-post","status-publish","format-standard","has-post-thumbnail","","category-biology","category-virtual-labs"],"modified_by":"Muhamed Elmesery","_links":{"self":[{"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/posts\/1947","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/comments?post=1947"}],"version-history":[{"count":26,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/posts\/1947\/revisions"}],"predecessor-version":[{"id":5437,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/posts\/1947\/revisions\/5437"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/media\/4382"}],"wp:attachment":[{"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/media?parent=1947"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/categories?post=1947"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/tags?post=1947"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}