{"id":2128,"date":"2022-11-07T07:22:06","date_gmt":"2022-11-07T07:22:06","guid":{"rendered":"https:\/\/blog.praxilabs.com\/?p=2128"},"modified":"2025-10-19T18:48:00","modified_gmt":"2025-10-19T18:48:00","slug":"recrystallization","status":"publish","type":"post","link":"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/","title":{"rendered":"Recrystallization Definition, Principle &#038;Purpose"},"content":{"rendered":"<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_82_2 counter-hierarchy ez-toc-counter ez-toc-light-blue ez-toc-container-direction\">\r\n<div class=\"ez-toc-title-container\">\r\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\r\n<span class=\"ez-toc-title-toggle\"><\/span><\/div>\r\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#What_is_Recrystallization\" >What is Recrystallization?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#The_Purpose_of_Recrystallization\" >The Purpose of Recrystallization<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#The_Recrystallization_Principle\" >The Recrystallization Principle<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#Recrystallization_Temperature\" >Recrystallization Temperature<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#Recrystallization_Steps\" >Recrystallization Steps<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#1-_suitable_solvent_Detection\" >1- suitable solvent Detection<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#2-_Dissolving_the_Solute_in_the_Suitable_Solvent\" >2- Dissolving the Solute in the Suitable Solvent<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#3-_Cooling_the_Solution\" >3- Cooling the Solution<\/a><ul class='ez-toc-list-level-4' ><li class='ez-toc-heading-level-4'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#4-_Obtaining_the_Crystals_by_Filtration\" >4- Obtaining the Crystals by Filtration<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#5-_Allowing_the_Resulting_Crystals_to_Dry\" >5- Allowing the Resulting Crystals to Dry<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-11\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#Types_of_Impurities\" >Types of Impurities<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-12\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#How_Does_Recrystallization_Work_Acetanilide_Recrystallization\" >How Does Recrystallization Work? (Acetanilide Recrystallization)<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-13\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#Solubility_of_Acetanilide_in_Ethanol\" >Solubility of Acetanilide in Ethanol<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-14\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#Solubility_of_Acetanilide_in_Water\" >Solubility of Acetanilide in Water<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-15\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#Types_of_Recrystallization\" >Types of Recrystallization<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-16\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#1-_Single_Solvent_Recrystallization\" >1- Single Solvent Recrystallization<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-17\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#Multi-Solvent_Recrystallization\" >Multi-Solvent Recrystallization<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-18\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#Hot_Filtration_Recrystallization\" >Hot Filtration Recrystallization<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-19\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#Seeding\" >Seeding<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-20\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#Scratching_Glass_Container\" >Scratching Glass Container<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-21\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#Single_perfect_crystals\" >Single perfect crystals<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-22\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#_Applications_of_Recrystallization\" >\u00a0Applications of Recrystallization<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-23\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#Recrystallization_Revealed_Your_Top_FAQs_Answered\" >Recrystallization Revealed: Your Top FAQs Answered!<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-24\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#What_is_recrystallization_and_its_application\" >What is recrystallization and its application?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-25\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#What_are_the_7_steps_of_recrystallization\" >What are the 7 steps of recrystallization?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-26\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#What_is_the_principle_behind_recrystallization\" >What is the principle behind recrystallization?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-27\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#What_is_a_real_life_application_of_recrystallization\" >What is a real life application of recrystallization?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-28\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#What_are_the_types_of_recrystallization\" >What are the types of recrystallization?<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-29\" href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/11\/07\/recrystallization\/#Discover_Our_New_Release_Experiments_in_Chemistry\" >Discover Our New Release Experiments in Chemistry<\/a><\/li><\/ul><\/nav><\/div>\r\n<h2><span class=\"ez-toc-section\" id=\"What_is_Recrystallization\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>What is Recrystallization?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4867 size-full aligncenter\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-2.webp\" alt=\"Recrystallization \" width=\"225\" height=\"225\" srcset=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-2.webp 225w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-2-150x150.webp 150w\" sizes=\"auto, (max-width: 225px) 100vw, 225px\" \/><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The recrystallization definition is a process used for purifying compounds that contain impurities and these compounds are:<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Dissolved at solvents.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Solids at room temperature.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Obtained from <a href=\"https:\/\/praxilabs.com\/en\/blog\/2022\/06\/26\/9-vital-chemistry-laws-and-formulas\/\">chemical reactions<\/a> or natural sources.\u00a0\u00a0<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">On the other hand, the impurities include mixtures of soluble, insoluble, and colored substances. Each\u00a0 type of these impurities needs to be removed by a separate procedure in the recrystallization process to obtain a pure compound.<\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">As we know what is recrystallization , we will also know in this article the purpose of the recrystallization experiment, types of crystallization, working principle, examples and steps of crystallization, what are the properties of the solvent used in recrystallization, important applications and more.<\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"The_Purpose_of_Recrystallization\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>The Purpose of Recrystallization<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The purpose of recrystallization is to separate or remove the impurities in a solid compound that are dissolved in a solvent to obtain a pure compound. And this technique depends on the fact &#8220;Solid materials tend to be more soluble in hot liquids than in cold liquids.\u201d <\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"The_Recrystallization_Principle\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>The Recrystallization Principle<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The Recrystallization principle depends on solubility: compounds with impurities (called solutes) tend to be more soluble in hot liquids (<a href=\"https:\/\/en.wikipedia.org\/wiki\/Solvent\" target=\"_blank\" rel=\"noopener\">solvents<\/a>) than they are in cold solvents.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">In the case of a hot saturated solution, if it is allowed to cool, the solute (compound) is no longer soluble in the solvent and the formation of pure compound crystal starts. The impurities are dissolved and excluded from the crystals to form pure solid crystals that can be separated by the process of filtration.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The process of recrystallization can start as a result of:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Drop in\u00a0 temperature.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Seeding which means adding a pure crystal.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Scratching the wall of a glass container may lead to a minute crystal appearance that\u00a0 is called &#8220;nucleation&#8221;.<\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">The difference between crystals may depend on t<\/span><span style=\"font-weight: 400;\">he environmental conditions, experience, cooling rate, and the solvent nature.<\/span><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>In PraxiLabs <a href=\"https:\/\/praxilabs.com\/en\/virtual-chemistry-lab\">virtual chemistry lab<\/a>s, you can instantly track performance and guide science lab simulation practice<\/b><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b><strong><a class=\"maxbutton-3 maxbutton\" href=\"https:\/\/praxilabs.com\/en\/pricing\"><span class='mb-text'>Pick The Best Virtual Plan from Praxilabs<\/span><\/a><\/strong><\/b><\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Recrystallization_Temperature\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Recrystallization <\/b><b>Temperature<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">It is the temperature when the material begins to change its mechanical properties because of the new grains created\u00a0 at the grain boundaries of the already existing grains.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">In general, the recrystallization temperature is typically one-third to one-half the melting point, and raises the atomic mobility so the recrystallization begins.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The Recrystallization temperature is about 0.3-0.4 times the pure metals melting point and 0.5 times the\u00a0 alloys melting point.<\/span><\/p>\n<h2><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4868 size-full\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-3.webp\" alt=\"Recrystallization Steps\" width=\"420\" height=\"120\" srcset=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-3.webp 420w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-3-300x86.webp 300w\" sizes=\"auto, (max-width: 420px) 100vw, 420px\" \/><\/span><\/h2>\n<p>&nbsp;<\/p>\n<h2><span class=\"ez-toc-section\" id=\"Recrystallization_Steps\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Recrystallization Steps<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Note that in this example, we assume that the present impurities are the soluble ones only.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The 5 main steps of recrystallization procedure are:<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"1-_suitable_solvent_Detection\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>1- suitable solvent Detection<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Before carrying out the actual steps of the recrystallization process, you must determine the suitable solvent for the impure compound (solute) by doing the solubility tests.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Any compound usually exhibits one behavior of the general three:-<\/span><\/p>\n<ol>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Compound with high solubility in both cold and hot solvents.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Compound with low solubility in both cold and hot solvents.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"> Compound with low solubility in a cold solvent and high solubility in a hot solvent.<\/span><\/li>\n<\/ol>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Solvents that show the first two behaviors are not suitable for the recrystallization process. The suitable and optimal solvent that can be used as a recrystallization solvent is the one that exhibits the third behavior, that is, low soluble (or insoluble) at decreased temperatures, high soluble in high temperatures, and sparingly soluble at room temperature.<\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>The following diagram shows the relation between solubility and temperature<\/b><\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4869 size-full\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-4.webp\" alt=\"The following diagram shows the relation between solubility and temperature\" width=\"678\" height=\"311\" srcset=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-4.webp 678w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-4-300x138.webp 300w\" sizes=\"auto, (max-width: 678px) 100vw, 678px\" \/><\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"2-_Dissolving_the_Solute_in_the_Suitable_Solvent\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>2-<\/b> <b>Dissolving the Solute in the Suitable Solvent<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0The next step after determining the suitable solvent is adding the solvent (that is near or at boiling point) to a beaker containing the compound with the impurities. Then, heat the beaker and keep adding the solvent until the solute is dissolved completely.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"3-_Cooling_the_Solution\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>3- Cooling the Solution<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0In this step, we will allow the solution to cool slowly, first in the open air, and then allow it to continue cooling in an ice bath. The slow cooling here is important to obtain purer crystals that will precipitate at the bottom of the beaker (The slower the cooling rate, the larger the formed crystals).<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Note: The process of &#8220;seeding&#8221; means the addition of the compound\u2019s pure crystals that forms a surface for the solute for the recrystallization process.<\/span><\/p>\n<h4><span class=\"ez-toc-section\" id=\"4-_Obtaining_the_Crystals_by_Filtration\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>4- Obtaining the Crystals by Filtration<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h4>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0The important and desirable part of the mixture is the pure crystals that form at the bottom of the solution, and so the crystals must be collected and isolated from the solvent by filtration.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">&#8220;Vacuum filtration&#8221; is the common technique used to isolate the remaining crystals in the beaker. By using an aspirator, the suction is created and the process of filtration is done. By using a Buchner funnel, anything remaining in the beaker is poured.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">&#8220;Gravity filtration&#8221; can be used if there are no crystals seen in the beaker. Gravity filtration depends on adding activated carbon to the solution, then letting the new mixture (activated carbon and the solution) boil and by a funnel system, the new mixture is transferred to a new beaker that contains the boiling solvent. The excess carbon is removed by the filter paper in the funnel. After the slow cooling, large crystals should be formed and present at the bottom of the beaker.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"5-_Allowing_the_Resulting_Crystals_to_Dry\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>5- Allowing the Resulting Crystals to Dry<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>\u00a0<\/b><span style=\"font-weight: 400;\">After rinsing the crystals with a small amount of ice-cold solvent, we will let the crystals dry by-<\/span><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">1- Leaving them in the aspirator.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">2-\u00a0 Transferring them to a glass dish to wait a while longer.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Note: We can test the crystal&#8217;s purity by conducting a &#8220;melting point determination&#8221; test.<\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Types_of_Impurities\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><strong>Types of Impurities<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">There are three different types of impurities:<\/span><\/p>\n<ul>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Insoluble Impurities.<\/span><\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Colored Impurities.<\/span><\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Soluble Impurities.<\/span><\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0Now, we will clarify how to remove each type of the impurities:<\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Insoluble Impurities: <\/b><span style=\"font-weight: 400;\">This type can be removed easily by dissolving the compound in a hot solvent, filtering the solution to remove the insoluble impurities (as they are left behind in the filter paper), then evaporating the solvent to obtain the solid compound.<\/span><\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Colored impurities:<\/b><span style=\"font-weight: 400;\"> This type can be removed by the same steps in the case of insoluble impurities, but with an additional step.<\/span><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The additional step is adding activated charcoal after the compound is dissolved in the solvent. The role of activated charcoal here is to absorb the colored impurities, then it is left behind in the filter paper.<\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Soluble Impurities: <\/b><span style=\"font-weight: 400;\">This type can&#8217;t be removed by filtration, because its solubility is similar to the solubility of the desired compound.<\/span><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The soluble impurities can be removed by the following steps:<\/span><\/p>\n<ol>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Conducting the solubility tests to determine the suitable solvent (low solubility in cold solvent and high solubility in hot solvent).<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Dissolving the desired compound with the soluble impurities in a minimum of near or at boiling solvent.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Cooling the new solution slowly and without interruption. As a result of cooling the solution, the solubility of (the compound + the soluble impurities) decreases, so the solution becomes saturated with the desired compound, and the process of crystallization begins.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Only the desired compound crystals form as crystal formation is a highly selective process. On the other hand, the soluble impurities will remain in the solution even after the solution has cooled because they are found in smaller amounts and so the solution is never saturated with the impurities.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">After removing the solvent and the soluble impurities, a final rinse with an ice-cold solvent is done to clean off any residual soluble impurities.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Finally, after evaporating the solvent, the pure crystals of the desired compound are present.<\/span><\/li>\n<\/ol>\n<h2><span class=\"ez-toc-section\" id=\"How_Does_Recrystallization_Work_Acetanilide_Recrystallization\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>How Does Recrystallization Work? (Acetanilide Recrystallization)<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">For more understanding, we will take acetanilide recrystallization and recrystallization of acetophenetidin as examples of how recrystallization work.<\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-4870 size-medium aligncenter\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-5-300x162.webp\" alt=\"acetanilide formula\" width=\"300\" height=\"162\" srcset=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-5-300x162.webp 300w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-5.webp 305w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The following example depends on the fact that &#8220;Solid materials tend to be more soluble in hot liquids than in cold liquids.&#8221;<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Solubility_of_Acetanilide_in_Ethanol\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Solubility of Acetanilide in Ethanol<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">At 0 temperature, acetanilide in ethanol solubility is 18g\/100mL and this means at 0 \u00baC, if we put 50 grams of acetanilide in 100 mL of ethanol, about 18 grams will dissolve in the ethanol and about 32g (the rest) will remain suspended in the solution.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Notice that if we increase the temperature, the solubility also increases.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">At 50 \u00baC, acetanilide in ethanol solubility is 80g\/100mL and this means at 50 \u00baC (we increase the temperature of the same during the acetanilide-ethanol suspension) all of the acetanilide (80 grams) will dissolve in the ethanol. And we can add more acetanilide (more than 80 g) but in this case, we will have a saturated solution as the additional solid of acetanilide would no longer dissolve.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The supersaturated solution is formed when the solution is allowed to cool again to 0 temperature, as more acetanilide is dissolved in ethanol.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Solubility_of_Acetanilide_in_Water\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Solubility of Acetanilide in Water<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">At 99 \u00b0C, if we dissolve 5 grams of acetanilide in 100 ml of water, and then let the solution cool to 10 \u00b0C, we will notice that:<\/span><\/p>\n<ul>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\"> \u00a0 \u00a0 \u00a0 \u00a0 <\/span><span style=\"font-weight: 400;\">4.5 g will form crystals (The recovery would be 90%).<\/span><\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\"> \u00a0 \u00a0 \u00a0 \u00a0 <\/span><span style=\"font-weight: 400;\">0.5 g will remain in the solution (The 10% remaining in the solution would be lost).<\/span><\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">In the case of recrystallization of acetophenetidin, the best solvent for acetophenetidin is water because, at boiling point, the solute is completely soluble, but when the solution is placed in an ice bath, the crystals reappear.<\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>PraxiLabs provides <a href=\"https:\/\/praxilabs.com\/en\/virtual-labs\">virtual labs<\/a> simulations in Chemistry, Physics and Biology that you can access anytime and anywhere<\/b><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong><a class=\"maxbutton-3 maxbutton\" href=\"https:\/\/praxilabs.com\/en\/sign-up\"><span class='mb-text'>Start The Virtual Chemistry Labs Experience Now!<\/span><\/a><\/strong><\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Types_of_Recrystallization\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Types of Recrystallization<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Depending on the condition for the solution\u00a0 and the required solid for recrystallization, there are many different types of recrystallization. Here are the 6 most common types:-<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"1-_Single_Solvent_Recrystallization\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>1- Single Solvent Recrystallization<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The first type we will talk about is single solvent recrystallization which is also known as &#8221; Normal recrystallization&#8221;.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The steps of recrystallization in the single solvent method are:<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">First, the compound (with the impurity) is solvated into a suitable solvent that should have the ability to dissolve both solute and impurity.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Then, increase the temperature until the saturation point is reached and let the solution cool down.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">At a certain temperature, the solute is no longer invisible and its solubility drops down. Finally, by cooling, the crystals start to form and appear clearly.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">In the case of ideal conditions, don&#8217;t worry about the impurities as their solubility never decreases due to the drop in temperature as the impurities are soluble in the suitable solvent. In reality, this process is repeated several times to achieve good purity.<\/span><\/p>\n<ol start=\"2\">\n<li>\n<h3><span class=\"ez-toc-section\" id=\"Multi-Solvent_Recrystallization\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b> Multi-Solvent Recrystallization<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<\/li>\n<\/ol>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Multi-solvent recrystallization refers to the process of recrystallization that involves more than one.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The steps of recrystallization in the multi-solvent method are:<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">At a certain temperature, the solution (solute and impurity) is prepared in the first solvent, assuming that each of\u00a0 the impurity and the solute are soluble in that solvent.<\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Then, the second solvent is added to the solution which makes the solvent able to <\/span><span style=\"font-weight: 400;\">either dissolve <\/span><span style=\"font-weight: 400;\">the <\/span><span style=\"font-weight: 400;\">solute or impurity. This separates impurities from desired solutes one way or the other.<\/span><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The principle and procedure of both the single-step recrystallization and the multi-step recrystallization processes are the same but the difference is adding a second solvent to the solution (that contains the first solvent, the impurities, and the solute).<\/span><\/p>\n<ol start=\"3\">\n<li>\n<h3><span class=\"ez-toc-section\" id=\"Hot_Filtration_Recrystallization\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b> Hot Filtration Recrystallization<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<\/li>\n<\/ol>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-4871 size-full\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-6.webp\" alt=\"Hot Filtration Recrystallization\" width=\"702\" height=\"155\" srcset=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-6.webp 702w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/07\/Recrystallization-Definition-6-300x66.webp 300w\" sizes=\"auto, (max-width: 702px) 100vw, 702px\" \/><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Hot filtration recrystallization is the type that is suitable in the case that\u00a0 insoluble impurities and other materials are present along with the desired solute. But here the simple recrystallization procedure is not enough to achieve the required purity.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The insoluble impurities must be filtered out when the mixture is in the form of a solution and the only thing filterable is the insoluble material. This process depends on using filter paper that serves the purpose but the filtration assembly should be kept warm to make sure that no crystallization of the desired solute happens.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Notice that if the solute were crystallized before the step of passing through the filter paper, it would be lost and the main purpose of the recrystallization procedure would fail.<\/span><\/p>\n<ol start=\"4\">\n<li>\n<h3><span class=\"ez-toc-section\" id=\"Seeding\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b> Seeding<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<\/li>\n<\/ol>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Seeding is considered an additional step to the already suitable recrystallization process. In this case, the saturated solution needs an induction to begin the process of crystallization which could be done by adding a seed.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">As we mentioned before, seeding means adding a pure crystal of the compound which forms a surface for the solute to crystallize. Only a single crystal of the desired solute or even a dust particle can be used for this purpose.<\/span><\/p>\n<ol start=\"5\">\n<li>\n<h3><span class=\"ez-toc-section\" id=\"Scratching_Glass_Container\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b> Scratching Glass Container<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<\/li>\n<\/ol>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Scratching glass container is the same as seeding. Scratching glass containers creates vibrations that may lead to the required arrangement of solute molecules together, which leads to stimulate crystallization.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">It is suggested that this process is a part of the seeding process because when scratching glass, the small particles of glass that wither away from the glass surface can serve as the sites of seeding and so it stimulates the nucleation to begin.<\/span><\/p>\n<ol start=\"6\">\n<li>\n<h3><span class=\"ez-toc-section\" id=\"Single_perfect_crystals\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b> Single perfect crystals<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<\/li>\n<\/ol>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Making perfect crystals has always been a difficult process. The small amounts of solute and the slow crystal development can help to grow and form perfect crystals. For this goal, some techniques are used like:<\/span><\/p>\n<ol>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Solvent slow evaporation.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Slow solvent diffusion by applying the mechanism of evaporation condensation to the second solvent (in case of multi-solvent recrystallization).<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Volatile solvent slow evaporation.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">In multistep recrystallization, applying interface delay for the 2 solvents to intermix.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">By solvent delivery or intermixing equipment that is specially designed.<\/span><\/li>\n<\/ol>\n<h2><span class=\"ez-toc-section\" id=\"_Applications_of_Recrystallization\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">\u00a0<\/span><b>Applications of Recrystallization<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">There are many useful applications of the recrystallization technique like:<\/span><\/p>\n<ul>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Purification for crystalline materials.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Pharmaceutical product development such as paracetamol is purified by the recrystallization process and also by studying the impact of crystal habit on these products.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Studying compounds&#8217; flowability and compaction in different temperatures and environments.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Studying the drug mechanism and regeneration.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Texture controlling for certain crystals.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Studying certain chemical compounds&#8217; wettability and bioavailability.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Isomers separation depending on the difference of solubility as a chemical property<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Synthesis of <a href=\"https:\/\/praxilabs.com\/en\/blog\/2018\/11\/29\/introduction-to-nanotechnology-and-its-most-important-applications-in-various-fields\/\">nanoparticles<\/a> involved in drug delivery systems.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Comparative study for the solubility of 2 or more solute components for chiral recognition and others.<\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Studying nucleation and compaction. <\/span><\/li>\n<li style=\"font-weight: 400;\" aria-level=\"1\"><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Polymorphism study, which means studying the ability of a crystal structure to exist in more than one crystal.<\/span><\/li>\n<\/ul>\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><strong>Try Praxilabs\u00a0 <a href=\"https:\/\/praxilabs.com\/en\/3d-science-simulations\">3D science Experiments<\/a> Lab for free now with over 45 different and unique experiments available.<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong><a class=\"maxbutton-3 maxbutton\" href=\"https:\/\/praxilabs.com\/en\/request-free-demo\"><span class='mb-text'>Request a Free Demo Now<\/span><\/a><\/strong><\/span><\/p>\n<h2 style=\"text-align: left;\"><span class=\"ez-toc-section\" id=\"Recrystallization_Revealed_Your_Top_FAQs_Answered\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Recrystallization Revealed: Your Top FAQs Answered!<\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<h3><span class=\"ez-toc-section\" id=\"What_is_recrystallization_and_its_application\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>What is recrystallization and its application?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Recrystallization is a process used for purifying compounds that contain impurities which are obtained from chemical reactions or natural sources.\u00a0 The main purpose of recrystallization is to separate or remove the impurities in a solid compound that are dissolved in a solvent to obtain a pure compound.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">One application is in\u00a0 pharmaceutical product development; such as paracetamol which is purified by the recrystallization process and also by studying the impact of crystal structure on these products.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"What_are_the_7_steps_of_recrystallization\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>What are the 7 steps of recrystallization?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>The 7 steps of recrystallization can be summarized as the following:<\/b><\/span><\/p>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong><span style=\"font-size: 14pt;\">1. Pick the solvent<\/span><\/strong><\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">The criteria used to choose an appropriate recrystallization solvent includes<\/span><span style=\"font-weight: 400;\"><br \/>\n<\/span><\/span><\/p>\n<ul>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Finding a solvent with a <\/span><i><span style=\"font-weight: 400;\">high temperature coefficient<\/span><\/i><span style=\"font-weight: 400;\">.\u00a0<\/span><\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Using a solvent that <\/span><i><span style=\"font-weight: 400;\">dissolves impurities readily or<\/span><\/i><span style=\"font-weight: 400;\"> does not dissolve them at all..\u00a0<\/span><\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Ensuring the solvent <\/span><i><span style=\"font-weight: 400;\">does not react with the solute<\/span><\/i><span style=\"font-weight: 400;\">.\u00a0<\/span><\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Using a solvent that is <\/span><i><span style=\"font-weight: 400;\">nonflammable, inexpensive and volatile<\/span><\/i><span style=\"font-weight: 400;\">.\u00a0 Solvents with low boiling points (i.e., volatile) can be easily removed from the resultant crystals by simply allowing the solvent to evaporate.\u00a0<\/span><\/span><\/li>\n<\/ul>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong><span style=\"font-size: 14pt;\">2. Dissolve the solute<\/span><\/strong><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0The solute should only dissolve when the solvent is heated. Therefore, the solvent is heated to its boiling point and then slowly added to completely dissolve the solute.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0Dissolving the solute generally involves adding a small volume of hot solvent, swirling the flask (or stirring the solution), and watching to see if the solute dissolves.<\/span><\/p>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong><span style=\"font-size: 14pt;\">3. Decolorize the solution<\/span><\/strong><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">If the solute is supposed to be white in its pure solid state (most organic solids are) and the solution is colored after dissolving all the solute, decolorizing carbon should be added.\u00a0 This step will help cause\u00a0 the colored molecules to adsorb onto the surface of the decolorizing carbon, thereby ridding the solution of these impurities.\u00a0 Should these impurities remain in solution, they may become trapped in the developing crystal during cooling.\u00a0 Review the material about decolorizing carbon.<\/span><\/p>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong><span style=\"font-size: 14pt;\">4. Filter any solids from the hot solution<\/span><\/strong><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">If decolorizing carbon was used or undissolved impurities remain in the hot solution, it is necessary to use gravity filtration while the solution\u00a0 is still hot. Do not use vacuum filtration with a Buchner funnel under any circumstances,as this can cause premature crystal formation while the solution passes through the vacuum filter.\u00a0<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">This leads to premature crystal development as the solution passes through the vacuum filter.\u00a0 (The vacuum reduces the pressure, but also the temperature.) If Impurities become trapped in the crystal lattice and steps 1 through 3 will need repeated!<\/span><\/p>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong><span style=\"font-size: 14pt;\">5. Crystallize the solute\u00a0<\/span><\/strong><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0This involves allowing the hot solution with the solute dissolved to return to room temperature slowly.\u00a0 The slower the cooling process, the lower the chance of trapping impurities in the developing crystal lattice. Allow the solution to reach room temperature.\u00a0<\/span><\/p>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong><span style=\"font-size: 14pt;\">6. Collect and wash the crystals\u00a0<\/span><\/strong><\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">The resultant crystals formed via this process can be collected by vacuum filtration, provided the solution is at room temperature and no further crystal growth is evident. To ensure all crystals transfer to the Buchner funnel., rinse with a small amount of the <\/span><b>cold<\/b><span style=\"font-weight: 400;\"> recrystallization solvent.\u00a0<\/span><\/span><\/p>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong><span style=\"font-size: 14pt;\">7. Dry the crystals<\/span><\/strong><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">After purification, it\u2019s usually necessary to quickly dry the crystals, especially if a melting point is to be measured immediately\u00a0 This can be done by leaving the crystals in the Buchner funnel and keeping the vacuum on for a few minutes.\u00a0 Alternatively, if more time is allotted, the crystals can be stored safely and allowed to sit for a few days, allowing the solvent to evaporate over time.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"What_is_the_principle_behind_recrystallization\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>What is the principle behind recrystallization?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">The main principle for recrystallization is based on dissolving the impure solid compound in hot solvent to form a saturated solution. As this solution cools, the solubility of the compound decreases, and pure crystals from the\u00a0 solution (the solubility of most solids increases with increased temperature).<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"What_is_a_real_life_application_of_recrystallization\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>What is a real life application of recrystallization?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Purification of seawater is a real life application of recrystallization.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Recrystallization is applied in the salt purification process to remove unwanted impurities, aiming to obtain ahight concentration of sodium chloride (NaCl) while minimizing impurities as far as possible.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"What_are_the_types_of_recrystallization\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>What are the types of recrystallization?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Here are the most common types of recrystallization:-<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Single Solvent Recrystallization.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Multi-Solvent Recrystallization.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Hot Filtration Recrystallization.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Seeding.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Scratching Glass Container.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Single perfect crystals.<\/span><\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"Discover_Our_New_Release_Experiments_in_Chemistry\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Discover Our New Release Experiments in Chemistry<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Organic Chemistry<\/b><\/span><\/p>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Grignrad Preparation<\/b><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">By the end of Grignrad preparation, your students will be able to:<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Become\u00a0 proficient at running organic chemical reactions.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Learn the basics of Grignard preparation.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Understand the mechanism of Grignard reactions.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Recognize the function of Grignard reactions.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Gain hands-on training on how the setup of reaction is used.<\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Preparation of Ethyl Propionate (Propanoic acid ethyl ester) (Fisher Esterification)<\/b><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Preparation of Ethyl Propionate (Propanoic acid ethyl ester) (Fisher Esterification) simulation will help your students to:<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Become proficient at running organic chemical reactions.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Learn the basics of organic synthesis procedures.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Understand the mechanism of the Fischer Esterification reaction.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Recognize the purpose of the Fischer Esterification reaction.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Gain practical training on how reflux and the reaction setup are used.<\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Nitration of Methylbenzoate<\/b><\/span><\/p>\n<p><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">By the end of Nitration of Methylbenzoate simulation, your students will be able to:<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Become proficient at running organic chemical reactions<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Learn the basics of organic synthesis procedures.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Understand the mechanism of electrophilic nitration reactions for aromatic rings.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Identify the role of Electrophilic substitution reactions.<\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Develop skills in synthetic organic chemistry techniques.<\/span><\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Basic Radical Tests<\/b><\/span><\/p>\n<ul>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Test for Unknown Basic Radical 13<\/span><\/li>\n<li><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\">Test for Unknown Basic Radical 14<\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>In our basic radical tests, students will <\/b><span style=\"font-weight: 400;\">understand various tests to identify the cation present in a given salt, and the chemical reactions that take place during each test. They will also acquire the skills to perform the experiment in a real lab once they understand the different steps in the procedure.<\/span><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b>Visit Our<\/b><a href=\"https:\/\/praxilabs.com\/en\/3d-science-simulations\"> <b>Ever-Expanding Catalog<\/b><\/a><b> of 3D Chemistry Virtual Simulations and Enhance Your Students\u2019 Learning Outcomes!<\/b><span style=\"font-weight: 400;\">\u00a0<\/span><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>What is Recrystallization? The recrystallization definition is a process used for purifying compounds that contain impurities and these compounds are: Dissolved at solvents. Solids at room temperature. Obtained from chemical reactions or natural sources.\u00a0\u00a0 On the other hand, the impurities include mixtures of soluble, insoluble, and colored substances. Each\u00a0 type of these impurities needs to &hellip;<\/p>\n","protected":false},"author":8,"featured_media":4600,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_lmt_disableupdate":"no","_lmt_disable":"no","footnotes":""},"categories":[3],"tags":[],"class_list":["post-2128","post","type-post","status-publish","format-standard","has-post-thumbnail","","category-chemistry"],"modified_by":"Muhamed Elmesery","_links":{"self":[{"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/posts\/2128","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/comments?post=2128"}],"version-history":[{"count":15,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/posts\/2128\/revisions"}],"predecessor-version":[{"id":5443,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/posts\/2128\/revisions\/5443"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/media\/4600"}],"wp:attachment":[{"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/media?parent=2128"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/categories?post=2128"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/tags?post=2128"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}