{"id":3424,"date":"2025-02-22T19:32:34","date_gmt":"2025-02-22T19:32:34","guid":{"rendered":"https:\/\/praxilabs.com\/en\/blog\/?p=3424"},"modified":"2025-10-26T12:26:26","modified_gmt":"2025-10-26T12:26:26","slug":"online-pcr-simulation","status":"publish","type":"post","link":"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/","title":{"rendered":"Online PCR Simulation for Students : Ease to Learn"},"content":{"rendered":"<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Polymerase chain reaction is a fundamental procedure used for DNA amplification in several fields such as molecular biology research, forensic science, diagnostics, and more!<\/span><\/span><\/p>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">In this blog post we will learn more about the online PCR simulation , its benefits, types of online PCR simulation at PraxiLabs, and the advantages of choosing PraxiLabs online simulations.<\/span><\/p>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3435\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/02\/Types-of-online-pcr-simulation-at-Praxilabs.png\" alt=\"What is Polymerase Chain Reaction\" width=\"1000\" height=\"667\" srcset=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/02\/Types-of-online-pcr-simulation-at-Praxilabs.png 1000w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/02\/Types-of-online-pcr-simulation-at-Praxilabs-300x200.png 300w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/02\/Types-of-online-pcr-simulation-at-Praxilabs-768x512.png 768w\" sizes=\"auto, (max-width: 1000px) 100vw, 1000px\" \/><\/span><\/p>\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_84 counter-hierarchy ez-toc-counter ez-toc-light-blue ez-toc-container-direction\">\r\n<div class=\"ez-toc-title-container\">\r\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">Table of Contents<\/p>\r\n<span class=\"ez-toc-title-toggle\"><\/span><\/div>\r\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#What_is_Polymerase_Chain_Reaction\" >What is Polymerase Chain Reaction?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#Real-time_PCR_Vs_Conventional_PCR\" >Real-time PCR Vs Conventional PCR<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#What_is_the_Purpose_of_PCR_Activities\" >What is the Purpose of PCR Activities?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-4\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#Benefits_of_Online_PCR_Simulation\" >Benefits of Online PCR Simulation<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-5\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#Cost_Effective\" >Cost Effective<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-6\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#Safe_and_Controlled_Environment\" >Safe and Controlled Environment<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-7\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#Time_Effective\" >Time Effective<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-8\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#Suitable_for_Teaching_and_Training\" >Suitable for Teaching and Training<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-9\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#Unlimited_Time_to_Experiment\" >Unlimited Time to Experiment<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-10\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#Explore_the_Types_of_Online_PCR_Simulation_at_PraxiLabs\" >Explore the Types of Online PCR Simulation at PraxiLabs<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-11\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#Real-Time_PCR_simulator\" >Real-Time PCR simulator<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-12\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#Conventional_PCR_Virtual_Simulation\" >Conventional PCR Virtual Simulation<\/a><\/li><\/ul><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-13\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#Frequently_Asked_Questions\" >Frequently Asked Questions<\/a><ul class='ez-toc-list-level-3' ><li class='ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-14\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#Are_virtual_PCR_labs_useful_for_educational_purposes\" >Are virtual PCR labs useful for educational purposes?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-15\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#What_equipment_is_simulated_in_a_real-time_PCR_simulator\" >What equipment is simulated in a real-time PCR simulator?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-16\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#Can_I_use_a_virtual_PCR_lab_on_my_mobile_device\" >Can I use a virtual PCR lab on my mobile device?<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-3'><a class=\"ez-toc-link ez-toc-heading-17\" href=\"https:\/\/praxilabs.com\/en\/blog\/2025\/02\/22\/online-pcr-simulation\/#How_can_I_access_the_virtual_PCR_labs\" >How can I access the virtual PCR labs?<\/a><\/li><\/ul><\/li><\/ul><\/nav><\/div>\r\n<h2><span class=\"ez-toc-section\" id=\"What_is_Polymerase_Chain_Reaction\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif; font-size: 14pt;\"><b>What is Polymerase Chain Reaction?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Polymerase chain reaction or PCR is a widespread biotechnological technique that is used for amplifying millions to billions of copies of a specific DNA segment repeatedly, in order to study it in more detail.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">PCR involves using short synthetic DNA fragments called primers to select a segment of the genome to be amplified, and then multiple rounds of DNA synthesis to replicate that segment.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">There are three basic steps in PCR amplification:<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Template denaturation (separating double-stranded DNA).<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Primer annealing to each original strand for new strand synthesis.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Extension of the new DNA strands from the primers.<\/span><\/li>\n<\/ul>\n<h2><span class=\"ez-toc-section\" id=\"Real-time_PCR_Vs_Conventional_PCR\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif; font-size: 14pt;\"><b>Real-time PCR Vs Conventional PCR<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Let\u2019s compare Real-time PCR and\u00a0 Conventional PCR<\/span><\/p>\n<table style=\"width: 100%;\">\n<tbody>\n<tr>\n<td style=\"width: 14.4578%;\"><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0<\/span><\/td>\n<td style=\"width: 41.2651%;\">\n<p style=\"text-align: center;\"><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong>Real-time PCR<\/strong><\/span><\/p>\n<\/td>\n<td style=\"width: 43.3734%;\">\n<p style=\"text-align: center;\"><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong>Conventional PCR<\/strong><\/span><\/p>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center; width: 14.4578%;\"><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong>Advantages<\/strong><\/span><\/td>\n<td style=\"width: 41.2651%;\">\n<ul>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Much faster and less time-consuming.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Decrease the chances of contamination.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">More sensitive than the conventional PCR.<\/span><\/li>\n<\/ul>\n<\/td>\n<td style=\"width: 43.3734%;\">\n<ul>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">More cost effective and cheaper than real time PCR.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Simpler and easier to use.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">No specialized training required.<\/span><\/li>\n<\/ul>\n<\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center; width: 14.4578%;\"><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong>Procedure<\/strong><\/span><\/td>\n<td style=\"width: 41.2651%;\"><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">\u00a0In\u00a0 Real-time PCR procedure, amplicons (final products)\u00a0 are stained with <\/span><b>fluorescent <\/b><span style=\"font-weight: 400;\">dye and measured at each cycle which allows the monitoring of the progress of the PCR reaction as it occurs; hence called Real- time PCR.<\/span><\/span><\/td>\n<td style=\"width: 43.3734%;\"><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">\u00a0Conventional PCR does not monitor the amplification process in real time but instead detects the amplicons (final products) using <\/span><b>gel electrophoresis<\/b><span style=\"font-weight: 400;\">\u00a0 only after the reaction is complete.<\/span><\/span><\/td>\n<\/tr>\n<tr>\n<td style=\"text-align: center; width: 14.4578%;\"><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong>Detection and Quantification<\/strong><\/span><\/td>\n<td style=\"width: 41.2651%;\"><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0In Real-time PCR product is measured at each cycle.<\/span><\/td>\n<td style=\"width: 43.3734%;\"><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0The detection and quantification of the amplified sequence occurs at the end of the reaction after the last PCR cycle.<\/span><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<h2><span class=\"ez-toc-section\" id=\"What_is_the_Purpose_of_PCR_Activities\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif; font-size: 14pt;\"><b>What is the Purpose of PCR Activities?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">The purpose of PCR is the amplification of a specific segment of a nucleic acid. It has several applications such as:<\/span><\/p>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Forensic scientists use PCR to connect blood, saliva, or tissue left at the scene of a crime to a suspect or victim.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Clinical geneticists use PCR to determine whether or not potential parents might carry a genetic disease that could be passed on to their children<\/span><\/p>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-3436 aligncenter\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/02\/online-pcr-simulation-for-students.png\" alt=\"Benefits of Online PCR Simulation\" width=\"900\" height=\"550\" srcset=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/02\/online-pcr-simulation-for-students.png 900w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/02\/online-pcr-simulation-for-students-300x183.png 300w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/02\/online-pcr-simulation-for-students-768x469.png 768w\" sizes=\"auto, (max-width: 900px) 100vw, 900px\" \/><\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Benefits_of_Online_PCR_Simulation\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif; font-size: 14pt;\"><b>Benefits of Online PCR Simulation<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<h3><span class=\"ez-toc-section\" id=\"Cost_Effective\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif; font-size: 12pt;\"><b>Cost Effective<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">In a real physical lab, the reagents and equipment are expensive. A virtual PCR simulation eliminates these requirements and makes it more suitable for educational institutions.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Safe_and_Controlled_Environment\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif; font-size: 12pt;\"><b>Safe and Controlled Environment<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Online polymerase chain reaction simulation provides a safe, controlled, and risk-free environment, reducing the chances of contamination, lab accidents, and several other risks.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Time_Effective\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif; font-size: 12pt;\"><b>Time Effective<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Conducting a PCR experiment using virtual labs saves a lot of time.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">For example:<\/span><\/p>\n<ul>\n<li><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">In a real lab, the total duration required for a <a href=\"https:\/\/praxilabs.com\/en\/3d-simulations\/real-time-pcr-virtual-lab-simulation\">real time PCR<\/a> experiment is about 2 hours. But, in virtual labs, it requires only eight minutes!<\/span><\/span><\/li>\n<li><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">For conventional PCR in a real lab, it takes about three hours, but in the virtual lab, it takes only about ten minutes!<\/span><\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\"><a href=\"https:\/\/praxilabs.com\/\">Virtual lab Simulation<\/a>\u00a0also contribute to saving the time spent to preparing the equipment and materials required to conduct the experiments<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Suitable_for_Teaching_and_Training\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif; font-size: 12pt;\"><b>Suitable for Teaching and Training<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Educators can rely on PCR virtual simulation to demonstrate the principles and protocols to their students clearly, without the need for physical reagents, tools, and equipment.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Unlimited_Time_to_Experiment\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif; font-size: 12pt;\"><b>Unlimited Time to Experiment<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Students can repeat PCR simulation experiments many times and can even speed up the process when necessary. Additionally, students can perform the experiment anytime and anywhere at their own pace.<\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong>Increase your Students\u2019 Learning Retention and Engagement with PraxiLabs\u2019 virtual labs!<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><b><a class=\"maxbutton-3 maxbutton\" href=\"https:\/\/praxilabs.com\/en\/request-free-demo\"><span class='mb-text'>Request A Free Demo Now!<\/span><\/a><\/b><\/span><\/p>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3433\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/02\/online-PCR-simulation.webp\" alt=\"Explore the Types of Online PCR Simulation at PraxiLabs\" width=\"900\" height=\"550\" srcset=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/02\/online-PCR-simulation.webp 900w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/02\/online-PCR-simulation-300x183.webp 300w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2025\/02\/online-PCR-simulation-768x469.webp 768w\" sizes=\"auto, (max-width: 900px) 100vw, 900px\" \/><\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Explore_the_Types_of_Online_PCR_Simulation_at_PraxiLabs\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif; font-size: 14pt;\"><b>Explore the Types of Online PCR Simulation at PraxiLabs<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">PraxiLabs provides two types of online PCR simulations:<\/span><\/p>\n<h3><\/h3>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3016\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2024\/08\/Dive-into-Real-Time-PCR-with-PraxiLabs-Virtual-Lab.jpg\" alt=\"Hands-On Learning: Dive into Real Time PCR with PraxiLabs!\" width=\"1200\" height=\"795\" srcset=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2024\/08\/Dive-into-Real-Time-PCR-with-PraxiLabs-Virtual-Lab.jpg 1200w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2024\/08\/Dive-into-Real-Time-PCR-with-PraxiLabs-Virtual-Lab-300x199.jpg 300w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2024\/08\/Dive-into-Real-Time-PCR-with-PraxiLabs-Virtual-Lab-1024x678.jpg 1024w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2024\/08\/Dive-into-Real-Time-PCR-with-PraxiLabs-Virtual-Lab-768x509.jpg 768w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2024\/08\/Dive-into-Real-Time-PCR-with-PraxiLabs-Virtual-Lab-310x205.jpg 310w\" sizes=\"auto, (max-width: 1200px) 100vw, 1200px\" \/><\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Real-Time_PCR_simulator\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><b>Real-Time PCR simulator<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Help your students gain hands-on experience with the principle, techniques, and procedures of Real Time PCR.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Learn more about the Procedure of <a href=\"https:\/\/praxilabs.com\/en\/3d-simulations\/real-time-pcr-virtual-lab-simulation\" target=\"_blank\" rel=\"noopener\">real time PCR simulation<\/a> inside praxiLabs virtual lab:<\/span><\/p>\n<ol>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\"> Set up the reaction while wearing gloves at all times and label the lids of the Eppendorf tube.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\"> Prepare an amount of the Master Mix sufficient of six samples of the DNA by adding the specific reagents for the experiment into the master mix tube.<\/span><\/li>\n<\/ol>\n<ul>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">93 \u03bcl of deionized water (15.5*6).<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">150 \u03bcl of 2x SYBR Green (25*6).<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">30 \u03bcl of the 10X PCR buffer containing MgCl2 (5*6).<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">6 \u03bcl of the 10 Mm dNTPs (1*6). &#8211; 6 \u03bcl of the Forward primer(1*6).<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">6 \u03bcl of the Reverse primer (1*6).<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">3 \u03bcl of the Taq DNA polymerase (0.5*6).<\/span><\/li>\n<\/ul>\n<ol start=\"3\">\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\"> Add 49 \u03bcl of the Master Mix you just prepared to 1 \u03bcl of each DNA sample.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\"> Place the Eppendorf tubes in the Real-Time PCR device and set up the reaction as follows:<\/span><\/li>\n<\/ol>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Initialization denaturation at 95 degrees Celsius for 5 minutes for one cycle.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Multiplication cycles, which is divided into three consecutive steps:<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Denaturation step: at 95 degrees Celsius for 30 seconds, 40 cycles.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Annealing step: at 55 degrees Celsius for one minute, 40 cycles.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Elongation step: at 72 degrees Celsius for one minute, 40 cycles.<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">\u00a0Final elongation which is divided into three consecutive steps:<\/span><\/p>\n<ul>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">95 degree Celsius for one minute for one cycle.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">55 degrees Celsius for 30 seconds for one cycle.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">95 degrees Celsius for 30 seconds for one cycle.<\/span><\/li>\n<\/ul>\n<ol start=\"5\">\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\"> Run the device.<\/span><\/li>\n<li><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\"> After the end of the process check the results from the Mx 3000P software.<\/span><\/li>\n<\/ol>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-3017\" src=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2024\/08\/Discover-the-Conventional-PCR-Simulation-in-the-PraxiLabs-Virtual-Lab.jpg\" alt=\"From Theory to Practice: Conventional PCR in the PraxiLabs Virtual Lab!\" width=\"1200\" height=\"882\" srcset=\"https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2024\/08\/Discover-the-Conventional-PCR-Simulation-in-the-PraxiLabs-Virtual-Lab.jpg 1200w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2024\/08\/Discover-the-Conventional-PCR-Simulation-in-the-PraxiLabs-Virtual-Lab-300x221.jpg 300w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2024\/08\/Discover-the-Conventional-PCR-Simulation-in-the-PraxiLabs-Virtual-Lab-1024x753.jpg 1024w, https:\/\/praxilabs.com\/en\/blog\/wp-content\/uploads\/2024\/08\/Discover-the-Conventional-PCR-Simulation-in-the-PraxiLabs-Virtual-Lab-768x564.jpg 768w\" sizes=\"auto, (max-width: 1200px) 100vw, 1200px\" \/><\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Conventional_PCR_Virtual_Simulation\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif; font-size: 14pt;\"><b>Conventional PCR Virtual Simulation<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Teach your students the procedure and protocol of conventional <a href=\"https:\/\/praxilabs.com\/en\/3d-simulations\/conventional-pcr-biology-virtual-lab-simulation\" target=\"_blank\" rel=\"noopener\">PCR virtual lab<\/a> , let them practice the main laboratory techniques included in the experiments.<\/span><\/p>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">By the end of the experiment, they will understand the role of specific reagents and equipment in PCR, and learn more about the applications of PCR.<\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong>PraxiLabs Virtual Labs include a range of online <a href=\"https:\/\/praxilabs.com\/en\/3d-science-simulations\">3D science experiments<\/a> in physics, chemistry and biology<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-size: 12pt; font-family: tahoma, arial, helvetica, sans-serif;\"><b><a class=\"maxbutton-3 maxbutton\" href=\"https:\/\/praxilabs.com\/en\/pricing\"><span class='mb-text'>Pick the Best Virtual Plan or You<\/span><\/a><\/b><\/span><\/p>\n<h2><span class=\"ez-toc-section\" id=\"Frequently_Asked_Questions\"><\/span><span style=\"font-size: 14pt; font-family: tahoma, arial, helvetica, sans-serif;\"><strong>Frequently Asked Questions<\/strong><\/span><span class=\"ez-toc-section-end\"><\/span><\/h2>\n<h3><span class=\"ez-toc-section\" id=\"Are_virtual_PCR_labs_useful_for_educational_purposes\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><b><\/b><span style=\"font-size: 12pt;\"><b> Are virtual PCR labs useful for educational purposes?<\/b><\/span><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Yes, as they are cost effective, time effective , and very safe. Many studies have proven the effectiveness of using <a href=\"https:\/\/praxilabs.com\/en\/virtual-labs\">virtual labs.<\/a><\/span><\/p>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">Virtual Labs Improve students outcomes:<\/span><\/span><\/p>\n<ul>\n<li><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">60% improvement\u00a0 in students&#8217; performance.<\/span><\/span><\/li>\n<li><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">80% increase in students&#8217; retention.<\/span><\/span><\/li>\n<\/ul>\n<h3><span class=\"ez-toc-section\" id=\"What_equipment_is_simulated_in_a_real-time_PCR_simulator\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><i><\/i><span style=\"font-size: 12pt;\"><b> What equipment is simulated in a real-time PCR simulator?<\/b><\/span><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><span style=\"font-weight: 400;\">\u00a0Several equipment, such as centrifuges, pipettes, vortex mixers, thermal cyclers, refrigerators, freezers, and analysis instruments.<\/span><\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"Can_I_use_a_virtual_PCR_lab_on_my_mobile_device\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><b><\/b><span style=\"font-size: 12pt;\"><b> Can I use a virtual PCR lab on my mobile device?<\/b><\/span><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\">Yes, PraxiLabs provides a mobile app that enables users to conduct experiments on their mobile phone with the feature \u201cOffline Mood- Download Experiment\u201d.<\/span><\/p>\n<h3><span class=\"ez-toc-section\" id=\"How_can_I_access_the_virtual_PCR_labs\"><\/span><span style=\"font-family: tahoma, arial, helvetica, sans-serif; font-size: 12pt;\"><b>How can I access the virtual PCR labs?<\/b><\/span><span class=\"ez-toc-section-end\"><\/span><\/h3>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Choose a trusted platform that provides virtual labs simulate pcr such as PraxiLabs<\/span><\/p>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">All you need to start our <a href=\"https:\/\/praxilabs.com\/en\/virtual-biology-lab\">virtual biology lab<\/a> simulations is\u00a0<\/span><\/p>\n<ul>\n<li><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong>Create a Free Account<\/strong><\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">No hardware setup. No credit card required. Get started in seconds.<\/span><\/p>\n<ul>\n<li><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong>Choose from 200+ Simulations<\/strong><\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Select three FREE Simulations and get started.<\/span><\/p>\n<ul>\n<li><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong>Practice as Many Times as You Want<\/strong><\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400; font-family: tahoma, arial, helvetica, sans-serif;\">Your lab partner will help you out, plus walkthrough videos, tutorials, &amp; more<\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><strong>Create your free account now and gain hands-on experience with the principle and practice of conventional PCR and real time PCR!<\/strong><\/span><\/p>\n<p style=\"text-align: center;\"><span style=\"font-family: tahoma, arial, helvetica, sans-serif;\"><b><a class=\"maxbutton-3 maxbutton\" href=\"https:\/\/praxilabs.com\/en\/sign-up\"><span class='mb-text'>Join Praxilabs For Free Now!<\/span><\/a><\/b><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Polymerase chain reaction is a fundamental procedure used for DNA amplification in several fields such as molecular biology research, forensic science, diagnostics, and more! In this blog post we will learn more about the online PCR simulation , its benefits, types of online PCR simulation at PraxiLabs, and the advantages of choosing PraxiLabs online simulations. &hellip;<\/p>\n","protected":false},"author":8,"featured_media":3434,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_lmt_disableupdate":"no","_lmt_disable":"no","footnotes":""},"categories":[1],"tags":[],"class_list":["post-3424","post","type-post","status-publish","format-standard","has-post-thumbnail","","category-virtual-learning"],"modified_by":"Muhamed Elmesery","_links":{"self":[{"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/posts\/3424","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/users\/8"}],"replies":[{"embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/comments?post=3424"}],"version-history":[{"count":12,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/posts\/3424\/revisions"}],"predecessor-version":[{"id":5463,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/posts\/3424\/revisions\/5463"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/media\/3434"}],"wp:attachment":[{"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/media?parent=3424"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/categories?post=3424"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/praxilabs.com\/en\/blog\/wp-json\/wp\/v2\/tags?post=3424"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}