{"id":944,"date":"2021-09-20T10:43:55","date_gmt":"2021-09-20T10:43:55","guid":{"rendered":"https:\/\/blog.praxilabs.com\/?p=944"},"modified":"2025-08-22T23:05:26","modified_gmt":"2025-08-22T23:05:26","slug":"elisa-principle","status":"publish","type":"post","link":"https:\/\/praxilabs.com\/en\/blog\/2021\/09\/20\/elisa-principle\/","title":{"rendered":"ELISA Principle, Procedure, Types, and Applications"},"content":{"rendered":"

The Enzyme Linked Immunosorbent Assay (ELISA) is one of the most sensitive immunoassays available. The typical detection range for an ELISA is 0.1 to 1 fmole or 0.01 ng to 0.1 ng. It is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies, and hormones.\u00a0<\/span><\/p>\n

In an ELISA, an antigen must be immobilized to a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme activity via incubation with a substrate to produce a measurable product. The most crucial element of the detection strategy is a highly specific antibody-antigen interaction.<\/span><\/p>\n

In this article we will discuss ELISA principle, procedure, types, and applications.<\/span><\/p>\n

Try ELISA Principle at our Virtual biology lab!<\/span><\/a><\/strong><\/span><\/p>\n

\"ELISA<\/span><\/p>\n

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