Biology

Last Updated on May 25, 2023 by Sara Assem DNA extraction is one of the basic techniques employed in the molecular laboratory, done for the first time by Friedrich Miescher in 1869. In this article, we will focus on DNA extraction methods, comparing methods for more understanding. Along with this, we will explain DNA extraction protocol, DNA extraction experiment, and …

Last Updated on April 11, 2023 by Sara Assem Try to mention the expression of genetic engineering in humans among a bunch of people, or a community gathering. Now try to escape the endless amount of questions, hurdles, and debates that will arise. technical  Since their first days, genetic engineering, gene modification, and traits selection have always been controversial and …

Last Updated on June 18, 2023 by Sara Assem ELISpot assay stands for the enzyme-linked immunospot assay. It is a very sensitive method used for measuring the number of cytokines secreting cells. And then measuring the antigen-specific T cells. In this article we will disscus ElISpot principle, steps, benifits, protocol, applications and more.   ELISpot Assay for T Cell Responses …

Last Updated on April 11, 2023 by Sara Assem The Enzyme Linked Immunosorbent Assay (ELISA) is one of the most sensitive immunoassays available. The typical detection range for an ELISA is 0.1 to 1 fmole or 0.01 ng to 0.1 ng. It is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies, and hormones.  In an ELISA, …

Last Updated on May 25, 2023 by Sara Assem Western blot is an indispensable mechanism in the modern biomedical research laboratory, as well as in laboratories doing research in other areas. It is considered as an analytical technique used mainly in molecular biology and immunogenetics where antibodies are used to specifically detect their antigen. Before discussing how to analyze western …