Biology

Last Updated on June 18, 2023 by Sara Assem ELISpot assay stands for the enzyme-linked immunospot assay. It is a very sensitive method used for measuring the number of cytokines secreting cells. And then measuring the antigen-specific T cells. In this article we will disscus ElISpot principle, steps, benifits, protocol, applications and more.   ELISpot Assay for T Cell Responses …

Last Updated on April 11, 2023 by Sara Assem The Enzyme Linked Immunosorbent Assay (ELISA) is one of the most sensitive immunoassays available. The typical detection range for an ELISA is 0.1 to 1 fmole or 0.01 ng to 0.1 ng. It is a plate-based assay technique designed for detecting and quantifying peptides, proteins, antibodies, and hormones.  In an ELISA, …

Last Updated on May 25, 2023 by Sara Assem Western blot is an indispensable mechanism in the modern biomedical research laboratory, as well as in laboratories doing research in other areas. It is considered as an analytical technique used mainly in molecular biology and immunogenetics where antibodies are used to specifically detect their antigen. Before discussing how to analyze western …

Last Updated on June 18, 2023 by Sara Assem Before talking about cell cycle regulation, we should know more about the term “Cell Cycle” and what it means. Definition of Cell Cycle Cell cycle is the process through which cells replicate and make two new cells. We can also define cell cycle as a series of events that takes place …

Last Updated on August 4, 2024 by Nourhan Essam Polymerase chain reaction (PCR) is now a common and often indispensable technique used in medical and biological research labs for a variety of applications. These include DNA cloning for sequencing, DNA-based phylogeny, or functional analysis of genes, the diagnosis of hereditary diseases, and the detection and diagnosis of infectious diseases. The …