- In the aspirin titration lab, students will analyze aspirin (acetylsalicylic acid) tablets “analgesic tablets” using the titration of aspirin experiment.Acetylsalicylic acid content will be assessed using a volumetric analysis technique.
- Aspirin tablets contains some inactive components such as binders e.g. starch that are added to hold tablet together during formulation, disintegrating agents e.g. cyclodextrin that are added to help in tablet break down after administration, and lubricants e.g. lactose.
- All of these are in addition to the active component which is acetyl salicylic acid. Sodium hydroxide could react with the acetylsalicylic acid but not with the other inactive ingredients such as starch binders, etc.
- According to the following reaction:
C 9 H 8 O 4 + Na OH → Na C 9 H 7 O 4 + H 2 O
acetylsalicylic acid sodium hydroxide sodium acetylsalicylate water
- This is an acid-base reaction in which the acetylsalicylic acid reacts with the base sodium hydroxide to produce the salt sodium acetylsalicylate and water (acid + base → salt + water).
- However, the aspirin titration experiment is preferably carried out as typical indirect or back titration. Standardized NaOH will be used to back titrate an aspirin solution and determine the concentration of aspirin in a typical analgesic tablet.
- Briefly, known excess NaOH will be added to known amount of aspirin, then the unreacted NaOH after completion of the reaction is back titrated with standardized HCl.
- Many reactions such as reaction between acetyl salicylic acid and sodium hydroxide are slow or present unfavorable equilibria for direct titration. Since, aspirin is a weak acid, therefore, it undergoes slow hydrolysis.
- As shown in Figure 1, each aspirin molecule reacts with two hydroxide ions. To overcome this problem, a known excess amount of base is added to the sample solution and an HCl titration is carried out to determine the amount of unreacted base.
- This is subtracted from the initial amount of base to find the amount of base that actually reacted with the aspirin and hence the quantity of aspirin in the analyte.
Figure 1. Reaction between acetyl salicylic acid and NaOH
- Since this is considered acid-base titration, phenolphthalein is an indicator as it changes color when all the unreacted NaOH has been reacted.
- This is called the “endpoint” of the reaction. The endpoint is detected when the color of phenolphthalein changes from pink to colorless.
In summary, the titration of aspirin allows accurate determination of the active ingredient in commercial tablets despite the presence of inactive components.