Home | Simulations
First Strand cDNA Synthesis

General Aim

To perform cDNA synthesis from RNA templates using reverse transcriptase enzyme.

 

Method

First Strand cDNA Synthesis for RT-PCR using Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Theoretical Background / Context

• cDNA Synthesis describes the generation of complementary DNA (cDNA) from an RNA template by reverse transcription. 
• Reverse transcriptases (RTase) use an RNA template and a primer complementary to the RNA to direct the synthesis of the first-strand cDNA, which can be used directly as a template for several processes.  
• Viruses synthesize cDNA so that they can be incorporated efficiently into the host chromosome. 

cDNA synthesis principle could be used in the following applications:

• Gene cloning.

• Creation of a cDNA library.

• DNA microarray.

• qPCR.

• Studying gene expressions

 

cdna Synthesis principle of Work

• cDNA Synthesis describes the generation of complementary DNA (cDNA) from an RNA template by reverse transcription in the virtual lab molecular biology.
• Reverse transcriptases (RTase) use an RNA template and a primer complementary to the RNA to direct the synthesis of the first strand cDNA, which can be used directly as a template for several processes.