First Strand cDNA Synthesis

Biology | Molecular Biology | Biochemistry | Genetics | Microbiology

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First Strand cDNA Synthesis

General Aim

To perform cDNA synthesis from RNA templates using reverse transcriptase enzyme.

 

Method

First Strand cDNA Synthesis for RT-PCR using Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Learning Objectives (ILOs)

  • By the end of first strand cDNA synthesis simulation, student will learn to:

  • Demonstrate proficiency with the cdna preparation principle and protocol involved in it.
  • Identify the role of specific reagents and equipment in cDNA Synthesis.
  • Practice basic laboratory techniques.
  • Conclude downstream applications of cDNA Synthesis.

Theoretical Background / Context

  • cDNA Synthesis describes the generation of complementary DNA (cDNA) from an RNA template by reverse transcription. 
  • Reverse transcriptases (RTase) use an RNA template and a primer complementary to the RNA to direct the synthesis of the first-strand cDNA, which can be used directly as a template for several processes.  
  • Viruses synthesize cDNA so that they can be incorporated efficiently into the host chromosome. 

cDNA synthesis principle could be used in the following applications:

• Gene cloning.

• Creation of a cDNA library.

• DNA microarray.

• qPCR.

• Studying gene expressions

 

cdna Synthesis principle of Work

  • cDNA Synthesis describes the generation of complementary DNA (cDNA) from an RNA template by reverse transcription in the virtual lab molecular biology.
  • Reverse transcriptases (RTase) use an RNA template and a primer complementary to the RNA to direct the synthesis of the first strand cDNA, which can be used directly as a template for several processes.

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