ELISA Immunology Virtual Lab Simulation| PraxiLabs

elisa virtual lab

Biology | Immunology | Biochemistry

As Featured In

ELISA Experiment: What You Need to Know (H2)

Importance of ELISA Experiment

To detect and measure the concentration of an analyte (usually antibodies, antigens, hormones, proteins or peptides) in solutions.

ELISA Experiment Method

Sandwich ELISA.

ELISA Virtual Lab: Learning Objectives for Students

  • To practice the steps of a successful sandwich ELISA.

  • To explain the importance of the washing steps.

ELISA Experiment: Theoretical Background

ELISA begins with a coating step, where the first layer either an antigen or an antibody is absorbed to a polystyrene 96 well plate. Coating is followed by blocking and detection steps and finally reading the results on ELISA reader.

There are four basic ELISA formats, allowing for a certain amount of flexibility which can be adjusted based on the antibodies available, the results required, or the complexity of the samples:

  • Direct ELISA.
  • Indirect ELISA.
  • Sandwich ELISA.
  • Competition or Inhibition ELISA.

ELISA is one of the most sensitive immunoassays available. The typical detection range for an ELISA is 0.1 to 1 fmole or 0.01 ng to 0.1 ng.

Sandwich ELISA Uses

Sandwich ELISA uses are very important in many fields like: Food allergens identification Authenticity of the food products corroboration Vaccine Development Measuring and monitoring the changes of the immune response for understanding immune disease.


Advantages of Sandwich ELISA

The main advantages of Sandwich ELISA are: The sample does not have to be purified before analysis, so it is suitable for complex samples. Flexibility and sensitivity since both direct and indirect detection methods can be used. High specificity since the antigen is specifically detected and captured.


The Sandwich ELISA Principle of Work

The Enzyme Linked Immunosorbent Assay (ELISA) is a common laboratory technique which is used to measure the concentration of the analyte (usually antibodies or antigens) in solutions. The steps of the ELISA result in a colored end product which correlates to the amount of the analytes present in the original sample. In this experiment we will be performing Sandwich ELISA in order to detect the presence of an antigen. Sandwich ELISA typically requires the use of matched antibody pairs, where each antibody is specific for different, non-overlapping parts of the antigen molecule. The first antibody termed the capture antibody is coated to the plate. Next, the analyte or sample solution is added to the well. A second antibody layer, the detection antibody, follows this step in order to measure the concentration of the analyte. If the detection antibody is conjugated to an enzyme, then the assay is called a direct sandwich ELISA. If the detection antibody is unlabeled, then a second detection antibody will be needed resulting in an indirect sandwich ELISA.

PraxiLabs is Recognized Worldwide

Customers Love PraxiLabs

“With the onset of the COVID-19 pandemic, we found ourselves in a situation that forced us to act quickly to find the best solution available to provide our students with a quality molecular genetics laboratory experience.”

Korri Thorlacius, B.Sc.
Biology Laboratory Instructor
Biology Department
Kwantlen Polytechnic University

'' Although there are now several vendors offering virtual reality software for physics labs, there is only one that offers a realistic, I feel like I’m in a real lab, solution: PraxiLabs.''

Dr.‌ ‌William‌ ‌H.‌ ‌Miner,‌ ‌Jr.‌ ‌
Professor‌ ‌of‌ ‌Physics‌ ‌
Palm‌ ‌Beach‌ ‌State‌ ‌College‌ ‌
Boca‌ ‌Raton,‌ ‌FL‌

" PraxiLabs offered my students a chance to actively engage with the material. Instead of watching videos on a topic, they could virtually complete labs and realize the practical applications of class topics. This is a quality alternative to in-person labs."

Crys Wright
Teaching Assistant
Texas A&M University, USA

"Great user experience and impressive interaction, I am very pleased to have tried the simulations and will continue to do so."

Dr. Khaled M Goher
Lecturer in Biomedical Engineering
Aston University, UK

The #1 Science Virtual Labs used by Educational Institutions

Explore More Interactive 3D Virtual Simulations

Designed for Safety and Engagement

Find out how PraxiLabs keeps students engaged and improves learning outcomes