General Aim of Western Blot Simulation
To detect a specific protein in a sample using western blot technique during the western blot simulation.
Method of Western Blot Simulation
Electroblotting of proteins into nitrocellulose membrane in western blot virtual lab.
Learning Objectives (ILOs)
By the end of western blot simulation, student will be able:
To identify the theory behind western blot protocol.
To fully comprehend the steps of western blot test.
To design a complete western blot assat experiment.
To analyze the visualized western blot protein quantification.
Theoretical Background / Context
- A blot, in molecular biology, is the technique that involves the transfer of DNA, RNA, and proteins (immunoblot).
- Labeled probes or antibodies can bind blotted proteins and reveal them, making it easier to study them.from a gel to a membrane (nitrocellulose or PVDF).
- Proteins can be detected by western blot analysis
- Western blot analyse is a laboratory technique used to detect and quantify specific proteins within a biological sample.
Principle Work of Western Blot Simulation
- Western blot is a commonly used technique designed to study a specific protein in a sample containing many other proteins using the wet lab techniques list.
- In western blot simulation, samples are extracted then separated by gel electrophoresis (SDS-PAGE) according to their molecular weight.
- Then, the proteins are electrophoretically transferred (blotted) to a more durable surface (e.g. nitrocellulose membrane western blot or PVDF membrane) during western blot simulation.
- Finally, a specific antibody is used to detect the protein of interest during western blot animation.
- The bound and labeled protein can be visualized giving information about its molecular weight and relative quantity.
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