A blot, in molecular biology, is the technique that involves the transfer of DNA, RNA and proteins from a gel to a membrane (nitrocellulose or PVDF). Proteins can be detected by western blot (immunoblot). Labeled probes or antibodies can bind blotted proteins and reveal them, making it easier to study them.
The Difference between Elisa and Western Blot
The ELISA test uses enzymes or antibodies attached to a solid surface to create the test surface. A sample is then added to the test surface. Antibodies or enzymes that react or attach to proteins indicate a positive result. The Western blot test is performed after gel-electrophoresis. The separated proteins are transferred (or blotted) onto nitrocellulose or nylon membranes, which are then identified by specific antibodies that are tagged by a secondary protein. It detects viral antigens (proteins usually on the surface of viruses) using antibodies against those proteins. A positive Western blot indicates viral antigen presence.