Western Blot Simulation

Theoretical Background / Context

• A blot, in molecular biology, is the technique that involves the transfer of DNA, RNA, and proteins (immunoblot). 
• Labeled probes or antibodies can bind blotted proteins and reveal them, making it easier to study them.from a gel to a membrane (nitrocellulose or PVDF). 
• Proteins can be detected by western blot analysis
• Western blot analyse is a laboratory technique used to detect and quantify specific proteins within a biological sample.

Principle Work of Western Blot Simulation

• Western blot is a commonly used technique designed to study a specific protein in a sample containing many other proteins using the wet lab techniques list. 
• In western blot simulation, samples are extracted then separated by gel electrophoresis (SDS-PAGE) according to their molecular weight. 
• Then, the proteins are electrophoretically transferred (blotted) to a more durable surface (e.g. nitrocellulose membrane western blot or PVDF membrane) during western blot simulation. 
• Finally, a specific antibody is used to detect the protein of interest during western blot animation.
• The bound and labeled protein can be visualized giving information about its molecular weight and relative quantity.