cDNA Synthesis

What Is cDNA Synthesis General Aim?

cDNA synthesis from RNA templates using reverse transcriptase enzyme.

cDNA Synthesis Experiment Method

First Strand cDNA Synthesis for RT-PCR using Avian Myeloblastosis Virus (AMV) reverse transcriptase.

cDNA Synthesis Experiment: Theoretical Background

cDNA Synthesis describes the generation of complementary DNA (cDNA) from an RNA template by reverse transcription. Reverse transcriptases (RTase) use an RNA template and a primer complementary to the RNA to direct the synthesis of the first strand cDNA, which can be used directly as a template for several processes.  Viruses synthesize cDNA so that they can be incorporated efficiently into the host chromosome. 
cDNA could be used in the following applications:

• Gene cloning.
• Creation of a cDNA library.
• DNA microarray.
• qPCR.
• Studying gene expressions.
   

What Is cDNA Synthesis Experiment Principle of Work?

cDNA Synthesis describes the generation of complementary DNA (cDNA) from an RNA template by reverse transcription. Reverse transcriptases (RTase) use an RNA template and a primer complementary to the RNA to direct the synthesis of the first strand cDNA, which can be used directly as a template for several processes.